Inflammation-Mediated Regulation of MicroRNA Expression in Transplanted Pancreatic Islets

المؤلفون المشاركون

Doni, Marco
Podetta, Michele
Vargas, Nancy
Rosero, Samuel
Molano, R. Damaris
Bravo-Egana, Valia
Tsinoremas, Nicholas F.
Pileggi, Antonello
Ricordi, Camillo
Pastori, Ricardo L.
Klein, Dagmar
Jiang, Zhijie

المصدر

Journal of Transplantation

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-15، 15ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2012-05-10

دولة النشر

مصر

عدد الصفحات

15

التخصصات الرئيسية

الطب البشري

الملخص EN

Nonspecific inflammation in the transplant microenvironment results in β-cell dysfunction and death influencing negatively graft outcome.

MicroRNA (miRNA) expression and gene target regulation in transplanted islets are not yet well characterized.

We evaluated the impact of inflammation on miRNA expression in transplanted rat islets.

Islets exposed in vitro to proinflammatory cytokines and explanted syngeneic islet grafts were evaluated by miRNA arrays.

A subset of 26 islet miRNAs was affected by inflammation both in vivo and in vitro.

Induction of miRNAs was dependent on NF-κB, a pathway linked with cytokine-mediated islet cell death.

RT-PCR confirmed expression of 8 miRNAs.

The association between these miRNAs and mRNA target-predicting algorithms in genome-wide RNA studies of β-cell inflammation identified 238 potential miRNA gene targets.

Several genes were ontologically associated with regulation of insulin signaling and secretion, diabetes, and islet physiology.

One of the most activated miRNAs was miR-21.

Overexpression of miR-21 in insulin-secreting MIN6 cells downregulated endogenous expression of the tumor suppressor Pdcd4 and of Pclo, a Ca2+ sensor protein involved in insulin secretion.

Bioinformatics identified both as potential targets.

The integrated analysis of miRNA and mRNA expression profiles revealed potential targets that may identify molecular targets for therapeutic interventions.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Bravo-Egana, Valia& Rosero, Samuel& Klein, Dagmar& Jiang, Zhijie& Vargas, Nancy& Tsinoremas, Nicholas F.…[et al.]. 2012. Inflammation-Mediated Regulation of MicroRNA Expression in Transplanted Pancreatic Islets. Journal of Transplantation،Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-493458

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Bravo-Egana, Valia…[et al.]. Inflammation-Mediated Regulation of MicroRNA Expression in Transplanted Pancreatic Islets. Journal of Transplantation No. 2012 (2012), pp.1-15.
https://search.emarefa.net/detail/BIM-493458

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Bravo-Egana, Valia& Rosero, Samuel& Klein, Dagmar& Jiang, Zhijie& Vargas, Nancy& Tsinoremas, Nicholas F.…[et al.]. Inflammation-Mediated Regulation of MicroRNA Expression in Transplanted Pancreatic Islets. Journal of Transplantation. 2012. Vol. 2012, no. 2012, pp.1-15.
https://search.emarefa.net/detail/BIM-493458

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-493458