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In Vitro Mass Multiplication and Assessment of Genetic Stability of In Vitro Raised Artemisia absinthium L. Plants Using ISSR and SSAP Molecular Markers
المؤلفون المشاركون
Kour, G.
Kour, B.
Dhar, M. K.
Kaul, S.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-13
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
The present investigations were made attempting to develop a rapid, reliable, and reproducible in vitro regeneration protocol for Artemisia absinthium L., a medicinal plant of Kashmir Himalayas.
Out of several auxin-cytokinin combinations tested, Murashige and Skoog’s (MS) medium supplemented with 0.5 mgL−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mgL−1 kinetin (Kn) was found to be the best for the callus induction.
On the other hand, 4.5 mgL−1 6-benzylaminopurine (BAP) and 0.5 mgL−1 1-α-naphthaleneacetic acid (NAA) in the medium resulted in maximum shoot induction from the callus.
Similarly, BAP and NAA at a concentration of 1.5 mgL−1 and 0.5 mgL−1, respectively, proved to be the best for the multiple shoot induction from nodal explants.
Numerous shoots were obtained from nodal explants after third subculture.
In vitro rooting was maximum on medium containing indole-3-butyric acid (IBA) at 0.5 mgL−1.
The genetic stability of the in vitro raised plants of Artemisia absinthium was assessed using the intersimple sequence repeat (ISSR) and sequence-specific amplification polymorphism (SSAP) molecular markers.
Both markers were able to detect the somaclonal variations in the callus regenerated plants, while no variation was detected in the plants regenerated from the nodal explants.
SSAP has been found to be more useful in detection of variability as compared to ISSR molecular marker.
The results of present study concluded that the direct regeneration protocol will be useful for the production of true to type plants of this medicinally important plant.
This will go a long way in reducing the pressure on the natural populations for the secondary metabolite production, especially for extraction of essential oils.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kour, B.& Kour, G.& Kaul, S.& Dhar, M. K.. 2014. In Vitro Mass Multiplication and Assessment of Genetic Stability of In Vitro Raised Artemisia absinthium L. Plants Using ISSR and SSAP Molecular Markers. Advances in Botany،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-493806
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kour, B.…[et al.]. In Vitro Mass Multiplication and Assessment of Genetic Stability of In Vitro Raised Artemisia absinthium L. Plants Using ISSR and SSAP Molecular Markers. Advances in Botany No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-493806
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kour, B.& Kour, G.& Kaul, S.& Dhar, M. K.. In Vitro Mass Multiplication and Assessment of Genetic Stability of In Vitro Raised Artemisia absinthium L. Plants Using ISSR and SSAP Molecular Markers. Advances in Botany. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-493806
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-493806
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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