Serine 129 Phosphorylation of α-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies

المؤلفون المشاركون

Zhu, Lihong
Zhang, GuoHua
Tao, Enxiang
Wang, Chuanming
Bi, Wei
Shi, Qiaoyun
Liang, Yanran
Sun, Yuanlin

المصدر

ISRN Neurology

العدد

المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-6، 6ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2011-04-11

دولة النشر

مصر

عدد الصفحات

6

التخصصات الرئيسية

الطب البشري

الملخص EN

Intraneuronal depositions of α-synuclein have been implicated in the pathogenesis of Parkinsons's disease (PD).

Previous reports have identified the crosslinking between α-synuclein and tTG (tissue transglutaminase) in both PD patients and the cellular model.

However, no researches have been conducted to further investigate their interaction in physiological conditions.

To address this question, we generated the SH-SY5Y cell line which stably expressed the wild-type or mutant (Ser129Ala) α-synuclein.

After the treatment with okadaic acid, α-synuclein started forming aggregates upon the activation of tTG.

Coimmunoprecipitation assays revealed a decreased interaction of the mutant α-synuclein S129A with tTG compared with the wild-type α-synuclein.

Cells expressing the wild-type α-synuclein showed increased eosinophilic cytoplasmic inclusion bodies that resembled Lewy bodies compared with the mutant.

Double immunofluorescence staining confirmed the colocalization of the phosphorylated α-synuclein and the tTG in the cells.

The S129A mutant demonstrated a lesser degree of colocalization than the wild type.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Bi, Wei& Zhang, GuoHua& Sun, Yuanlin& Zhu, Lihong& Wang, Chuanming& Liang, Yanran…[et al.]. 2011. Serine 129 Phosphorylation of α-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies. ISRN Neurology،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-494354

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Bi, Wei…[et al.]. Serine 129 Phosphorylation of α-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies. ISRN Neurology No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-494354

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Bi, Wei& Zhang, GuoHua& Sun, Yuanlin& Zhu, Lihong& Wang, Chuanming& Liang, Yanran…[et al.]. Serine 129 Phosphorylation of α-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies. ISRN Neurology. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-494354

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-494354