Serine 129 Phosphorylation of α-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies
Joint Authors
Zhu, Lihong
Zhang, GuoHua
Tao, Enxiang
Wang, Chuanming
Bi, Wei
Shi, Qiaoyun
Liang, Yanran
Sun, Yuanlin
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Intraneuronal depositions of α-synuclein have been implicated in the pathogenesis of Parkinsons's disease (PD).
Previous reports have identified the crosslinking between α-synuclein and tTG (tissue transglutaminase) in both PD patients and the cellular model.
However, no researches have been conducted to further investigate their interaction in physiological conditions.
To address this question, we generated the SH-SY5Y cell line which stably expressed the wild-type or mutant (Ser129Ala) α-synuclein.
After the treatment with okadaic acid, α-synuclein started forming aggregates upon the activation of tTG.
Coimmunoprecipitation assays revealed a decreased interaction of the mutant α-synuclein S129A with tTG compared with the wild-type α-synuclein.
Cells expressing the wild-type α-synuclein showed increased eosinophilic cytoplasmic inclusion bodies that resembled Lewy bodies compared with the mutant.
Double immunofluorescence staining confirmed the colocalization of the phosphorylated α-synuclein and the tTG in the cells.
The S129A mutant demonstrated a lesser degree of colocalization than the wild type.
American Psychological Association (APA)
Bi, Wei& Zhang, GuoHua& Sun, Yuanlin& Zhu, Lihong& Wang, Chuanming& Liang, Yanran…[et al.]. 2011. Serine 129 Phosphorylation of α-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies. ISRN Neurology،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-494354
Modern Language Association (MLA)
Bi, Wei…[et al.]. Serine 129 Phosphorylation of α-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies. ISRN Neurology No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-494354
American Medical Association (AMA)
Bi, Wei& Zhang, GuoHua& Sun, Yuanlin& Zhu, Lihong& Wang, Chuanming& Liang, Yanran…[et al.]. Serine 129 Phosphorylation of α-Synuclein Cross-Links with Tissue Transglutaminase to Form Lewy Body-Like Inclusion Bodies. ISRN Neurology. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-494354
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-494354