Formulation Changes Affect Material Properties and Cell Behavior in HA-Based Hydrogels
المؤلفون المشاركون
Potekhina, Lydia
McIntosh, Kristen
Lawyer, Thomas
Clavijo, Cristian
Mann, Brenda K.
المصدر
International Journal of Cell Biology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-27
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
To develop and optimize new scaffold materials for tissue engineering applications, it is important to understand how changes to the scaffold affect the cells that will interact with that scaffold.
In this study, we used a hyaluronic acid- (HA-) based hydrogel as a synthetic extracellular matrix, containing modified HA (CMHA-S), modified gelatin (Gtn-S), and a crosslinker (PEGda).
By varying the concentrations of these components, we were able to change the gelation time, enzymatic degradation, and compressive modulus of the hydrogel.
These changes also affected fibroblast spreading within the hydrogels and differentially affected the proliferation and metabolic activity of fibroblasts and mesenchymal stem cells (MSCs).
In particular, PEGda concentration had the greatest influence on gelation time, compressive modulus, and cell spreading.
MSCs appeared to require a longer period of adjustment to the new microenvironment of the hydrogels than fibroblasts.
Fibroblasts were able to proliferate in all formulations over the course of two weeks, but MSCs did not.
Metabolic activity changed for each cell type during the two weeks depending on the formulation.
These results highlight the importance of determining the effect of matrix composition changes on a particular cell type of interest in order to optimize the formulation for a given application.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lawyer, Thomas& McIntosh, Kristen& Clavijo, Cristian& Potekhina, Lydia& Mann, Brenda K.. 2012. Formulation Changes Affect Material Properties and Cell Behavior in HA-Based Hydrogels. International Journal of Cell Biology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-494684
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lawyer, Thomas…[et al.]. Formulation Changes Affect Material Properties and Cell Behavior in HA-Based Hydrogels. International Journal of Cell Biology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-494684
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lawyer, Thomas& McIntosh, Kristen& Clavijo, Cristian& Potekhina, Lydia& Mann, Brenda K.. Formulation Changes Affect Material Properties and Cell Behavior in HA-Based Hydrogels. International Journal of Cell Biology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-494684
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-494684
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر