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Store-Operated Ca2+ Entry Does Not Control Proliferation in Primary Cultures of Human Metastatic Renal Cellular Carcinoma
المؤلفون المشاركون
Potenza, Duilio Michele
Saitta, Anna
Montagna, Daniela
Prestia, Martina
Glasnov, Toma N.
Ferulli, Federica
Guerra, Germano
Porta, Camillo
Bottino, Cinzia
Tanzi, Franco
Luinetti, Ombretta
Laforenza, Umberto
Rosti, Vittorio
Pedrazzoli, Paolo
Ganini, Carlo
Turin, Ilaria
Mosca, Alessandra
Dragoni, Silvia
Moccia, Francesco
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-19، 19ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-09
دولة النشر
مصر
عدد الصفحات
19
التخصصات الرئيسية
الملخص EN
Store-operated Ca2+ entry (SOCE) is activated following depletion of the inositol-1,4,5-trisphosphate (InsP3)-sensitive Ca2+ pool to regulate proliferation in immortalized cell lines established from either primary or metastatic lesions.
The molecular nature of SOCE may involve both Stim1, which senses Ca2+ levels within the endoplasmic reticulum (ER) Ca2+ reservoir, and a number of a Ca2+-permeable channels on the plasma membrane, including Orai1, Orai3, and members of the canonical transient receptor (TRPC1–7) family of ion channels.
The present study was undertaken to assess whether SOCE is expressed and controls proliferation in primary cultures isolated from secondary lesions of heavily pretreated metastatic renal cell carcinoma (mRCC) patients.
SOCE was induced following pharmacological depletion of the ER Ca2+ store, but not by InsP3-dependent Ca2+ release.
Metastatic RCC cells express Stim1-2, Orai1–3, and TRPC1–7 transcripts and proteins.
In these cells, SOCE was insensitive to BTP-2, 10 µM Gd3+ and Pyr6, while it was inhibited by 100 µM Gd3+, 2-APB, and carboxyamidotriazole (CAI).
Neither Gd3+ nor 2-APB or CAI impaired mRCC cell proliferation.
Consistently, no detectable Ca2+ signal was elicited by growth factor stimulation.
Therefore, a functional SOCE is expressed but does not control proliferation of mRCC cells isolated from patients resistant to multikinase inhibitors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Saitta, Anna& Mosca, Alessandra& Guerra, Germano& Rosti, Vittorio& Luinetti, Ombretta& Ganini, Carlo…[et al.]. 2014. Store-Operated Ca2+ Entry Does Not Control Proliferation in Primary Cultures of Human Metastatic Renal Cellular Carcinoma. BioMed Research International،Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-494862
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Saitta, Anna…[et al.]. Store-Operated Ca2+ Entry Does Not Control Proliferation in Primary Cultures of Human Metastatic Renal Cellular Carcinoma. BioMed Research International No. 2014 (2014), pp.1-19.
https://search.emarefa.net/detail/BIM-494862
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Saitta, Anna& Mosca, Alessandra& Guerra, Germano& Rosti, Vittorio& Luinetti, Ombretta& Ganini, Carlo…[et al.]. Store-Operated Ca2+ Entry Does Not Control Proliferation in Primary Cultures of Human Metastatic Renal Cellular Carcinoma. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-19.
https://search.emarefa.net/detail/BIM-494862
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-494862
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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