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Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock : Downstream Regulation of COX-2, IL-1β, TNF-α, IL-6, and HMGB1 Expression
المؤلفون المشاركون
Sampaio, André L. F.
Wheatley, Carmen
Dalli, Jesmond
Perretti, Mauro
Brancaleone, Vincenzo
D'Acquisto, Fulvio
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-20، 20ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-05-28
دولة النشر
مصر
عدد الصفحات
20
التخصصات الرئيسية
الملخص EN
Background.
NOS/•NO inhibitors are potential therapeutics for sepsis, yet they increase clinical mortality.
However, there has been no in vivo investigation of the (in vitro) •NO scavenger, cobalamin’s (Cbl) endogenous effects on NOS/•NO/inflammatory mediators during the immune response to sepsis.
Methods.
We used quantitative polymerase chain reaction (qPCR), ELISA, Western blot, and NOS Griess assays, in a C57BL/6 mouse, acute endotoxaemia model.
Results.
During the immune response, pro-inflammatory phase, parenteral hydroxocobalamin (HOCbl) treatment partially inhibits hepatic, but not lung, iNOS mRNA and promotes lung eNOS mRNA, but attenuates the LPS hepatic rise in eNOS mRNA, whilst paradoxically promoting high iNOS/eNOS protein translation, but relatively moderate •NO production.
HOCbl/NOS/•NO regulation is reciprocally associated with lower 4 h expression of TNF-α, IL-1β, COX-2, and lower circulating TNF-α, but not IL-6.
In resolution, 24 h after LPS, HOCbl completely abrogates a major late mediator of sepsis mortality, high mobility group box 1 (HMGB1) mRNA, inhibits iNOS mRNA, and attenuates LPS-induced hepatic inhibition of eNOS mRNA, whilst showing increased, but still moderate, NOS activity, relative to LPS only.
experiments (LPS+D-Galactosamine) HOCbl afforded significant, dose-dependent protection in mice Conclusions.
HOCbl produces a complex, time- and organ-dependent, selective regulation of NOS/•NO during endotoxaemia, corollary regulation of downstream inflammatory mediators, and increased survival.
This merits clinical evaluation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sampaio, André L. F.& Dalli, Jesmond& Brancaleone, Vincenzo& D'Acquisto, Fulvio& Perretti, Mauro& Wheatley, Carmen. 2013. Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock : Downstream Regulation of COX-2, IL-1β, TNF-α, IL-6, and HMGB1 Expression. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-20.
https://search.emarefa.net/detail/BIM-495082
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sampaio, André L. F.…[et al.]. Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock : Downstream Regulation of COX-2, IL-1β, TNF-α, IL-6, and HMGB1 Expression. Mediators of Inflammation No. 2013 (2013), pp.1-20.
https://search.emarefa.net/detail/BIM-495082
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sampaio, André L. F.& Dalli, Jesmond& Brancaleone, Vincenzo& D'Acquisto, Fulvio& Perretti, Mauro& Wheatley, Carmen. Biphasic Modulation of NOS Expression, Protein and Nitrite Products by Hydroxocobalamin Underlies Its Protective Effect in Endotoxemic Shock : Downstream Regulation of COX-2, IL-1β, TNF-α, IL-6, and HMGB1 Expression. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-20.
https://search.emarefa.net/detail/BIM-495082
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-495082
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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