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Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis
المؤلفون المشاركون
Kumar, H.
Singh, S. V.
Singh, P. K.
Singh, A. V.
Sohal, J. S.
المصدر
Veterinary Medicine International
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-11-16
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Efficacy of IS900 blood PCR was evaluated for the presence of MAP infection.
Serum, fecal, and blood samples of kids, young, and adult goats from farm and farmer's herds in Mathura district were also screened by ELISA, microscopy and culture.
Of 111 goats (kids: 40, young: 14, adults: 57) screened, 77.5% were positive by blood PCR.
Of 76 goats, 90.8% (kids: 87.5% and adults: 94.4%) were positive by PCR.
From 21 kids and 14 young goats, 42.8 and 57.1% were positive.
gDNA from goats was genotyped as MAP “Indian Bison type”.
Of 21 fecal samples of kids examined by microscopy, 66.7% were positive.
In ELISA, 9.5 and 57.1% kids were positives as “type I” and “type II” reactors, respectively.
Screening 14 young goats by culture of blood clots, 28.6% were positive.
Agreement was substantial between PCR and microscopy.
It was fair and moderate when PCR and microscopy were compared with type I and type II reactors, respectively.
Presence of MAP in non-clinical kids and young goats indicate early or subclinical infection.
Blood PCR was rapid, sensitive, and specific assay for detection of MAP in any stage (early, subclinical, and clinical) and age (kids, young, and adult) of goats.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Singh, P. K.& Singh, S. V.& Kumar, H.& Sohal, J. S.& Singh, A. V.. 2009. Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis. Veterinary Medicine International،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-495601
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Singh, P. K.…[et al.]. Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis. Veterinary Medicine International No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-495601
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Singh, P. K.& Singh, S. V.& Kumar, H.& Sohal, J. S.& Singh, A. V.. Diagnostic Application of IS900 PCR Using Blood as a Source Sample for the Detection of Mycobacterium avium Subspecies Paratuberculosis in Early and Subclinical Cases of Caprine Paratuberculosis. Veterinary Medicine International. 2009. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-495601
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-495601
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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