Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro
المؤلفون المشاركون
Nolte, Ingo
Willenbrock, S.
Samy, M. T.
Wefstaedt, P.
Wagner, S.
Sterenczak, K. A.
Abd El-Aal, A. M.
Murua Escobar, H.
Ismail, A. A.
المصدر
Veterinary Medicine International
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-02-08
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Multipotency and self-renewal are considered as most important features of stem cells to persist throughout life in tissues.
In this context, the role of HMGA proteins to influence proliferation of adipose-derived mesenchymal stem cell (ASCs) while maintaining their multipotent and self-renewal capacities has not yet been investigated.
Therefore, extracellular HMGA1 and HMGA2 application alone (10–200 ng/mL) and in combination with each other (100, 200 ng/mL each) was investigated with regard to proliferative effects on canine ASCs (cASCs) after 48 hours of cultivation.
Furthermore, mRNA expression of multipotency marker genes in unstimulated and HMGA2-stimulated cASCs (50, 100 ng/mL) was analyzed by RT-qPCR.
HMGA1 significantly reduced cASCs proliferation in concentrations of 10–200 ng/mL culture medium.
A combination of HMGA1 and HMGA2 protein (100 and 200 ng/mL each) caused the same effects, whereas no significant effect on cASCs proliferation was shown after HMGA2 protein application alone.
RT-qPCR results showed that expression levels of marker genes including KLF4, SOX2, OCT4, HMGA2, and cMYC mRNAs were on the same level in both HMGA2-protein-stimulated and -unstimulated cASCs.
Extracellular HMGA protein application might be valuable to control proliferation of cASCs in context with their employment in regenerative approaches without affecting their self-renewal and multipotency abilities.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ismail, A. A.& Wagner, S.& Murua Escobar, H.& Willenbrock, S.& Sterenczak, K. A.& Samy, M. T.…[et al.]. 2012. Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro. Veterinary Medicine International،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-495918
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ismail, A. A.…[et al.]. Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro. Veterinary Medicine International No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-495918
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ismail, A. A.& Wagner, S.& Murua Escobar, H.& Willenbrock, S.& Sterenczak, K. A.& Samy, M. T.…[et al.]. Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro. Veterinary Medicine International. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-495918
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-495918
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر