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Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro
المؤلفون المشاركون
Nolte, Ingo
Willenbrock, S.
Samy, M. T.
Wefstaedt, P.
Wagner, S.
Sterenczak, K. A.
Abd El-Aal, A. M.
Murua Escobar, H.
Ismail, A. A.
المصدر
Veterinary Medicine International
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-02-08
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Multipotency and self-renewal are considered as most important features of stem cells to persist throughout life in tissues.
In this context, the role of HMGA proteins to influence proliferation of adipose-derived mesenchymal stem cell (ASCs) while maintaining their multipotent and self-renewal capacities has not yet been investigated.
Therefore, extracellular HMGA1 and HMGA2 application alone (10–200 ng/mL) and in combination with each other (100, 200 ng/mL each) was investigated with regard to proliferative effects on canine ASCs (cASCs) after 48 hours of cultivation.
Furthermore, mRNA expression of multipotency marker genes in unstimulated and HMGA2-stimulated cASCs (50, 100 ng/mL) was analyzed by RT-qPCR.
HMGA1 significantly reduced cASCs proliferation in concentrations of 10–200 ng/mL culture medium.
A combination of HMGA1 and HMGA2 protein (100 and 200 ng/mL each) caused the same effects, whereas no significant effect on cASCs proliferation was shown after HMGA2 protein application alone.
RT-qPCR results showed that expression levels of marker genes including KLF4, SOX2, OCT4, HMGA2, and cMYC mRNAs were on the same level in both HMGA2-protein-stimulated and -unstimulated cASCs.
Extracellular HMGA protein application might be valuable to control proliferation of cASCs in context with their employment in regenerative approaches without affecting their self-renewal and multipotency abilities.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ismail, A. A.& Wagner, S.& Murua Escobar, H.& Willenbrock, S.& Sterenczak, K. A.& Samy, M. T.…[et al.]. 2012. Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro. Veterinary Medicine International،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-495918
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ismail, A. A.…[et al.]. Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro. Veterinary Medicine International No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-495918
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ismail, A. A.& Wagner, S.& Murua Escobar, H.& Willenbrock, S.& Sterenczak, K. A.& Samy, M. T.…[et al.]. Effects of High-Mobility Group A Protein Application on Canine Adipose-Derived Mesenchymal Stem Cells In Vitro. Veterinary Medicine International. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-495918
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-495918
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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