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[6]-Gingerol Prevents Disassembly of Cell Junctions and Activities of MMPs in Invasive Human Pancreas Cancer Cells through ERKNF-κBSnail Signal Transduction Pathway
المؤلفون المشاركون
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-24
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
To study the effects of [6]-gingerol, a ginger phytochemical, on tight junction (TJ) molecules, we investigated TJ tightening and signal transduction pathways in human pancreatic duct cell-derived cancer cell line PANC-1.
The following methods were utilized: MTT assay to determine cytotoxicity; zymography to examine matrix metalloproteinase (MMP) activities; transepithelial electrical resistance (TER) and paracellular flux for TJ measurement; RT-PCR and immunoblotting for proteins related to TJ and invasion; and EMSA for NF-κB activity in PANC-1 cells.
Results revealed that TER significantly increased and claudin 4 and MMP-9 decreased compared to those of the control.
TJ protein levels, including zonula occludens (ZO-) 1, occludin, and E-cadherin, increased in [6]-gingerol-treated cells, which correlated with a decrease in paracellular flux and MMP activity.
Furthermore, NF-κB/Snail nuclear translocation was suppressed via downregulation of the extracellular signal-regulated kinase (ERK) pathway in response to [6]-gingerol treatment.
Moreover, treatment with U0126, an ERK inhibitor, completely blocked NF-κB activity.
In conclusion, these findings demonstrate that [6]-gingerol regulates TJ-related proteins and suppresses invasion and metastasis through NF-κB/Snail inhibition via inhibition of the ERK pathway.
Therefore, [6]-gingerol may suppress the invasive activity of PANC-1 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Sung Ok& Kim, Mi Ryeo. 2013. [6]-Gingerol Prevents Disassembly of Cell Junctions and Activities of MMPs in Invasive Human Pancreas Cancer Cells through ERKNF-κBSnail Signal Transduction Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-496737
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Sung Ok& Kim, Mi Ryeo. [6]-Gingerol Prevents Disassembly of Cell Junctions and Activities of MMPs in Invasive Human Pancreas Cancer Cells through ERKNF-κBSnail Signal Transduction Pathway. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-496737
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Sung Ok& Kim, Mi Ryeo. [6]-Gingerol Prevents Disassembly of Cell Junctions and Activities of MMPs in Invasive Human Pancreas Cancer Cells through ERKNF-κBSnail Signal Transduction Pathway. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-496737
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-496737
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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