The Study of the Frequency Effect of Dynamic Compressive Loading on Primary Articular Chondrocyte Functions Using a Microcell Culture System
المؤلفون المشاركون
Chang, Yu-Han
Yang, Tzu-Chi
Huang, Song-Bin
Wu, Min-Hsien
Lin, Wan-Ying
Chiu, Tzu-Keng
Wang, Hsin-Yao
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-16
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Compressive stimulation can modulate articular chondrocyte functions.
Nevertheless, the relevant studies are not comprehensive.
This is primarily due to the lack of cell culture apparatuses capable of conducting the experiments in a high throughput, precise, and cost-effective manner.
To address the issue, we demonstrated the use of a perfusion microcell culture system to investigate the stimulating frequency (0.5, 1.0, and 2.0 Hz) effect of compressive loading (20% and 40% strain) on the functions of articular chondrocytes.
The system mainly integrates the functions of continuous culture medium perfusion and the generation of pneumatically-driven compressive stimulation in a high-throughput micro cell culture system.
Results showed that the compressive stimulations explored did not have a significant impact on chondrocyte viability and proliferation.
However, the metabolic activity of chondrocytes was significantly affected by the stimulating frequency at the higher compressive strain of 40% (2 Hz, 40% strain).
Under the two compressive strains studied, the glycosaminoglycans (GAGs) synthesis was upregulated when the stimulating frequency was set at 1 Hz and 2 Hz.
However, the stimulating frequencies explored had no influence on the collagen production.
The results of this study provide useful fundamental insights that will be helpful for cartilage tissue engineering and cartilage rehabilitation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lin, Wan-Ying& Chang, Yu-Han& Wang, Hsin-Yao& Yang, Tzu-Chi& Chiu, Tzu-Keng& Huang, Song-Bin…[et al.]. 2014. The Study of the Frequency Effect of Dynamic Compressive Loading on Primary Articular Chondrocyte Functions Using a Microcell Culture System. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-496799
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lin, Wan-Ying…[et al.]. The Study of the Frequency Effect of Dynamic Compressive Loading on Primary Articular Chondrocyte Functions Using a Microcell Culture System. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-496799
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lin, Wan-Ying& Chang, Yu-Han& Wang, Hsin-Yao& Yang, Tzu-Chi& Chiu, Tzu-Keng& Huang, Song-Bin…[et al.]. The Study of the Frequency Effect of Dynamic Compressive Loading on Primary Articular Chondrocyte Functions Using a Microcell Culture System. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-496799
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-496799
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر