A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue
المؤلفون المشاركون
Larkö, Eva
Hakonen, Bodil
Lönnberg, Linnea K.
Blom, Kristina
المصدر
International Journal of Biomaterials
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-18
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
The lack of predictable in vitro methods to analyze antimicrobial activity could play a role in the development of resistance to antibiotics.
Current used methods analyze planktonic cells but for the method to be clinically relevant, biofilm in in vivo like conditions ought to be studied.
Hence, our group has developed a qualitative and quantitative method with in vivo like 3D tissue for prediction of antimicrobial activity in reality.
Devices (wound dressings) were applied on top of Pseudomonas aeruginosa inoculated Muller-Hinton (MH) agar or 3D synthetic soft tissues (SST) and incubated for 24 hours.
The antibacterial activity was then analyzed visually and by viable counts.
On MH agar two out of three silver containing devices showed zone of inhibitions (ZOI) and on SST, ZOI were detected for all three.
Corroborating results were found upon evaluating the bacterial load in SST and shown to be silver concentration dependent.
In conclusion, a novel method was developed combining visual rapid screening and quantitative evaluation of the antimicrobial activity in both tissue and devices.
It uses tissue allowing biofilm formation thus mimicking reality closely.
These conditions are essential in order to predict antimicrobial activity of medical devices in the task to prevent device related infections.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hakonen, Bodil& Lönnberg, Linnea K.& Larkö, Eva& Blom, Kristina. 2014. A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue. International Journal of Biomaterials،Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-497208
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hakonen, Bodil…[et al.]. A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue. International Journal of Biomaterials No. 2014 (2014), pp.1-5.
https://search.emarefa.net/detail/BIM-497208
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hakonen, Bodil& Lönnberg, Linnea K.& Larkö, Eva& Blom, Kristina. A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue. International Journal of Biomaterials. 2014. Vol. 2014, no. 2014, pp.1-5.
https://search.emarefa.net/detail/BIM-497208
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-497208
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر