The Critical Role of Redox Homeostasis in Shikonin-Induced HL-60 Cell Differentiation via Unique Modulation of the Nrf2ARE Pathway
المؤلفون المشاركون
Wang, Zhenhua
Zheng, Qiusheng
Zhang, Bo
Chen, Hongmei
Chen, Na
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-10-15
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
Among various cancer cell lines, the leukemia cell line HL-60 was most sensitive to Shikonin, with evidence showing both the prooxidative activities and proapoptotic effects of micromolar concentrations of Shikonin.
However, the mechanism involved in the cytotoxicity of Shikonin in the submicromolar range has not been fully characterized.
Using biochemical and free radical biological experiments in vitro, we identified the prodifferentiated profiles of Shikonin and evaluated the redox homeostasis during HL-60 differentiation.
The data showed a strong dose-response relationship between Shikonin exposure and the characteristics of HL-60 differentiation in terms of morphology changes, nitroblue tetrazolium (NBT) reductive activity, and the expression level of surface antigens CD11b/CD14.
During drug exposure, intercellular redox homeostasis changes towards oxidation are necessary to support Shikonin-induced differentiation, which was proven by additional enzymatic and non-enzymatic redox modulators.
A statistically significant and dose-dependent increase (P<0.05) was recorded with regard to the unique expression levels of the Nrf2/ARE downstream target genes in HL-60 cells undergoing late differentiation, which were restored with further antioxidants employed with the Shikonin treatment.
Our research demonstrated that Shikonin is a differentiation-inducing agent, and its mechanisms involve the Nrf2/ARE pathway to modulate the intercellular redox homeostasis, thus facilitating differentiation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Bo& Chen, Na& Chen, Hongmei& Wang, Zhenhua& Zheng, Qiusheng. 2012. The Critical Role of Redox Homeostasis in Shikonin-Induced HL-60 Cell Differentiation via Unique Modulation of the Nrf2ARE Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-497513
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Bo…[et al.]. The Critical Role of Redox Homeostasis in Shikonin-Induced HL-60 Cell Differentiation via Unique Modulation of the Nrf2ARE Pathway. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-497513
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Bo& Chen, Na& Chen, Hongmei& Wang, Zhenhua& Zheng, Qiusheng. The Critical Role of Redox Homeostasis in Shikonin-Induced HL-60 Cell Differentiation via Unique Modulation of the Nrf2ARE Pathway. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-497513
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-497513
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر