Effect of Rabbit Epididymal Antimicrobial Peptide, REHbβP, on LPS-Induced Proinflammatory Cytokine Responses in Human Vaginal Cells In Vitro
المؤلفون المشاركون
Sukanya, D.
Selvaakumar, C.
Reddy, K. V. R.
Patgaonkar, M. S.
المصدر
International Journal of Peptides
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-03-14
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Antimicrobial peptides (AMP’s) protect epithelial surfaces including epididymis against pathogens and play a key role in orchestrating various defensive responses.
Recently, we have identified one such AMP, rabbit epididymal hemoglobin-β subuit (REHbβP) from the epididymal fluid of rabbit, Oryctologus cuniculus.
The demonstration of a protective role of REHbβP in epididymal epithelial cells (EPEC’s) led us to investigate: (1) the identification of LPS interactive domain in REHbβP, and (2) whether the REHbβP of rabbit origin mediates vaginal cellular immune responses of another species (human).
HeLa-S3, human vaginal epithelial cells (hVECs) were exposed to LPS or the LPS-stimulated cells treated with REHbβP or neutral peptide, nREHbβP.
Effect of LPS and cytokines (IL-6 and IL-1α) and chemokines (IL-8, MCP-1) levels was determined in the culture supernatants.
In response to the LPS, hVECs synthesized these mediators and the levels were significantly higher than controls.
This enhancing effect was ameliorated when the LPS-induced hVECs were treated with REHbβP.
Similar results were obtained on NF-κB protein and hBD-1 mRNA expression.
Confocal microscopy studies revealed that REHbβP attenuated the LPS-induced internalization of E.
coli by macrophages.
The chemotaxis studies performed using Boyden chamber Transwell assay, which showed elevated migration of U937 cells when the supernatants of LPS-induced hVECs were used, and the effect was inhibited by REHbβP.
REHbβP was found to be localized on the acrosome of rabbit spermatozoa, suggesting its role in sperm protection beside sperm function.
In conclusion, REHbβP may have the potential to develop as a therapeutic agent for reproductive tract infections (RTI’s).
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Reddy, K. V. R.& Sukanya, D.& Patgaonkar, M. S.& Selvaakumar, C.. 2012. Effect of Rabbit Epididymal Antimicrobial Peptide, REHbβP, on LPS-Induced Proinflammatory Cytokine Responses in Human Vaginal Cells In Vitro. International Journal of Peptides،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-497572
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Reddy, K. V. R.…[et al.]. Effect of Rabbit Epididymal Antimicrobial Peptide, REHbβP, on LPS-Induced Proinflammatory Cytokine Responses in Human Vaginal Cells In Vitro. International Journal of Peptides No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-497572
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Reddy, K. V. R.& Sukanya, D.& Patgaonkar, M. S.& Selvaakumar, C.. Effect of Rabbit Epididymal Antimicrobial Peptide, REHbβP, on LPS-Induced Proinflammatory Cytokine Responses in Human Vaginal Cells In Vitro. International Journal of Peptides. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-497572
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-497572
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر