Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices
المؤلفون المشاركون
Guguen-Guillouzo, Christiane
Bichet, Nicole
Desmots, Fabienne
Masson, Régis
Finot, Francis
Vericat, Joan A.
Lafouge, Patricia
Ribault, Catherine
Loyer, Pascal
المصدر
International Journal of Hepatology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-10-16
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
The culture liver slices are mainly used to investigate drug metabolism and xenobiotic-mediated liver injuries while apoptosis and proliferation remain unexplored in this culture model.
Here, we show a transient increase in LDH release and caspase activities indicating an ischemic injury during the slicing procedure.
Then, caspase activities decrease and remain low in cultured slices demonstrating a low level of apoptosis.
The slicing procedure is also associated with the G0/G1 transition of hepatocytes demonstrated by the activation of stress and proliferation signalling pathways including the ERK1/2 and JNK1/2/3 MAPKinases and the transient upregulation of c-fos.
The cells further progress up to mid-G1 phase as indicated by the sequential induction of c-myc and p53 mRNA levels after the slicing procedure and at 24 h of culture, respectively.
The stimulation by epidermal growth factor induces the ERK1/2 phosphorylation but fails to activate expression of late G1 and S phase markers such as cyclin D1 and Cdk1 indicating that hepatocytes are arrested in mid-G1 phase of the cell cycle.
However, we found that combined stimulation by the proinflammatory cytokine tumor necrosis factor α and the epidermal growth factor promotes the commitment to DNA replication as observed in vivo during the liver regeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Finot, Francis& Masson, Régis& Desmots, Fabienne& Ribault, Catherine& Bichet, Nicole& Vericat, Joan A.…[et al.]. 2012. Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices. International Journal of Hepatology،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-497951
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Finot, Francis…[et al.]. Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices. International Journal of Hepatology No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-497951
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Finot, Francis& Masson, Régis& Desmots, Fabienne& Ribault, Catherine& Bichet, Nicole& Vericat, Joan A.…[et al.]. Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices. International Journal of Hepatology. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-497951
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-497951
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر