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Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices
المؤلفون المشاركون
Guguen-Guillouzo, Christiane
Bichet, Nicole
Desmots, Fabienne
Masson, Régis
Finot, Francis
Vericat, Joan A.
Lafouge, Patricia
Ribault, Catherine
Loyer, Pascal
المصدر
International Journal of Hepatology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-10-16
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
The culture liver slices are mainly used to investigate drug metabolism and xenobiotic-mediated liver injuries while apoptosis and proliferation remain unexplored in this culture model.
Here, we show a transient increase in LDH release and caspase activities indicating an ischemic injury during the slicing procedure.
Then, caspase activities decrease and remain low in cultured slices demonstrating a low level of apoptosis.
The slicing procedure is also associated with the G0/G1 transition of hepatocytes demonstrated by the activation of stress and proliferation signalling pathways including the ERK1/2 and JNK1/2/3 MAPKinases and the transient upregulation of c-fos.
The cells further progress up to mid-G1 phase as indicated by the sequential induction of c-myc and p53 mRNA levels after the slicing procedure and at 24 h of culture, respectively.
The stimulation by epidermal growth factor induces the ERK1/2 phosphorylation but fails to activate expression of late G1 and S phase markers such as cyclin D1 and Cdk1 indicating that hepatocytes are arrested in mid-G1 phase of the cell cycle.
However, we found that combined stimulation by the proinflammatory cytokine tumor necrosis factor α and the epidermal growth factor promotes the commitment to DNA replication as observed in vivo during the liver regeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Finot, Francis& Masson, Régis& Desmots, Fabienne& Ribault, Catherine& Bichet, Nicole& Vericat, Joan A.…[et al.]. 2012. Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices. International Journal of Hepatology،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-497951
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Finot, Francis…[et al.]. Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices. International Journal of Hepatology No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-497951
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Finot, Francis& Masson, Régis& Desmots, Fabienne& Ribault, Catherine& Bichet, Nicole& Vericat, Joan A.…[et al.]. Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices. International Journal of Hepatology. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-497951
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-497951
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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