E. coli-Derived L-Asparaginase Retains Enzymatic and Cytotoxic Activity In Vitro for Canine and Feline Lymphoma after Cold Storage
المؤلفون المشاركون
Wypij, Jackie M.
Pondenis, Holly C.
المصدر
Veterinary Medicine International
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-04-28
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background.
L-asparaginase is effective in treating canine and feline lymphoma, however chemotherapy poses a significant financial cost to veterinary clients, limiting therapy for many pets.
Single dose vials result in significant drug wastage, and drug shortages limit consistent availability for pets.
Hypothesis.
E.
coli-derived asparaginase retains enzymatic and antineoplastic activity in canine and feline lymphoma cells after cold storage.
Methods.
E.
coli-derived asparaginase was cold-stored: refrigeration (7–14 days) and freezing (14 days–six months, one to three freeze/thaw cycles).
Enzymatic activity of asparaginase was measured via a modified asparagine assay.
Effects of cold-stored asparaginase on cell proliferation and cytotoxicity were measured in feline (MYA-1, F1B) and canine (17–71, OSW) lymphoma cells.
Results.
Cold-stored E.
coli-derived asparaginase retains antineoplastic activity in all four cell lines tested.
Cold-stored E.
coli-derived L-asparaginase depletes asparagine and retains enzymatic activity.
Duration of refrigeration, duration of freezing, and number of freeze-thaw cycles have minimal effect on asparaginase enzyme activity.
Conclusions and Clinical Importance.
This study establishes a scientific basis for long-term cold storage of reconstituted E.
coli-derived asparaginase that may result in better utilization of limited drug resources and improve financial feasibility of E.
coli-derived asparaginase as a therapeutic option for pets with lymphoma.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wypij, Jackie M.& Pondenis, Holly C.. 2013. E. coli-Derived L-Asparaginase Retains Enzymatic and Cytotoxic Activity In Vitro for Canine and Feline Lymphoma after Cold Storage. Veterinary Medicine International،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-497980
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wypij, Jackie M.& Pondenis, Holly C.. E. coli-Derived L-Asparaginase Retains Enzymatic and Cytotoxic Activity In Vitro for Canine and Feline Lymphoma after Cold Storage. Veterinary Medicine International No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-497980
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wypij, Jackie M.& Pondenis, Holly C.. E. coli-Derived L-Asparaginase Retains Enzymatic and Cytotoxic Activity In Vitro for Canine and Feline Lymphoma after Cold Storage. Veterinary Medicine International. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-497980
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-497980
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر