Differences in Expression, Content, and Activity of 11β-HSD1 in Adipose Tissue between Obese Men and Women
المؤلفون المشاركون
Torres, A.
Mericq, Veronica
Ferrario, M.
Iñiguez, G.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-12-09
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Cortisol production in adipose tissue is regulated by 11β-HSD1.
Objective.
To determine whether there are differences in gene expression, enzyme activity, and protein content of the 11β-HSD1 enzyme in VAT (visceral adipose tissue) and SAT (subcutaneous adipose tissue) from obese compared to nonobese adults.
Methods.
VAT and SAT samples were obtained from 32 obese subjects (BMI>30 Kg/m2) who underwent bariatric surgery and 15 samples from controls submitted to elective surgery.
Fasting serum glucose, insulin, and lipids were measured.
The expression of 11β-HSD1 was determined by RT-PCR, the enzyme activity by thin-layer chromatography, and the protein content by Western blot.
Results.
Obese patients had higher cholesterol, insulin, and HOMA-IR compared to nonobese.
There were no differences in VAT or SAT expression of 11β-HSD1 between obese and nonobese patients.
However, we found lower 11β-HSD1 activity and protein content in VAT, in obese women versus nonobese women (P<0.05).
BMI and 11β-HSD1 enzyme activity and protein content in VAT correlated inversely in women.
Conclusions.
Regulation of 11β-HSD1 activity in VAT from obese subjects appears to be gender specific, suggesting the existence of a possible protective mechanism modulating this enzyme activity leading to a decrease in the production of cortisol in this tissue.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Torres, A.& Iñiguez, G.& Ferrario, M.& Mericq, Veronica. 2012. Differences in Expression, Content, and Activity of 11β-HSD1 in Adipose Tissue between Obese Men and Women. ISRN Endocrinology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-498080
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Torres, A.…[et al.]. Differences in Expression, Content, and Activity of 11β-HSD1 in Adipose Tissue between Obese Men and Women. ISRN Endocrinology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-498080
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Torres, A.& Iñiguez, G.& Ferrario, M.& Mericq, Veronica. Differences in Expression, Content, and Activity of 11β-HSD1 in Adipose Tissue between Obese Men and Women. ISRN Endocrinology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-498080
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-498080
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر