Differences in Expression, Content, and Activity of 11β-HSD1 in Adipose Tissue between Obese Men and Women
Joint Authors
Torres, A.
Mericq, Veronica
Ferrario, M.
Iñiguez, G.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Cortisol production in adipose tissue is regulated by 11β-HSD1.
Objective.
To determine whether there are differences in gene expression, enzyme activity, and protein content of the 11β-HSD1 enzyme in VAT (visceral adipose tissue) and SAT (subcutaneous adipose tissue) from obese compared to nonobese adults.
Methods.
VAT and SAT samples were obtained from 32 obese subjects (BMI>30 Kg/m2) who underwent bariatric surgery and 15 samples from controls submitted to elective surgery.
Fasting serum glucose, insulin, and lipids were measured.
The expression of 11β-HSD1 was determined by RT-PCR, the enzyme activity by thin-layer chromatography, and the protein content by Western blot.
Results.
Obese patients had higher cholesterol, insulin, and HOMA-IR compared to nonobese.
There were no differences in VAT or SAT expression of 11β-HSD1 between obese and nonobese patients.
However, we found lower 11β-HSD1 activity and protein content in VAT, in obese women versus nonobese women (P<0.05).
BMI and 11β-HSD1 enzyme activity and protein content in VAT correlated inversely in women.
Conclusions.
Regulation of 11β-HSD1 activity in VAT from obese subjects appears to be gender specific, suggesting the existence of a possible protective mechanism modulating this enzyme activity leading to a decrease in the production of cortisol in this tissue.
American Psychological Association (APA)
Torres, A.& Iñiguez, G.& Ferrario, M.& Mericq, Veronica. 2012. Differences in Expression, Content, and Activity of 11β-HSD1 in Adipose Tissue between Obese Men and Women. ISRN Endocrinology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-498080
Modern Language Association (MLA)
Torres, A.…[et al.]. Differences in Expression, Content, and Activity of 11β-HSD1 in Adipose Tissue between Obese Men and Women. ISRN Endocrinology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-498080
American Medical Association (AMA)
Torres, A.& Iñiguez, G.& Ferrario, M.& Mericq, Veronica. Differences in Expression, Content, and Activity of 11β-HSD1 in Adipose Tissue between Obese Men and Women. ISRN Endocrinology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-498080
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-498080