Structural Characterization of Amadori Rearrangement Product of Glucosylated Nα-Acetyl-Lysine by Nuclear Magnetic Resonance Spectroscopy
المؤلفون المشاركون
Li, Chuanjiang
Juárez, Manuel
Ruan, Eric Dongliang
Wang, Hui
المصدر
International Journal of Spectroscopy
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-17
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Maillard reaction is a nonenzymatic reaction between reducing sugars and free amino acid moieties, which is known as one of the most important modifications in food science.
It is essential to characterize the structure of Amadori rearrangement products (ARPs) formed in the early stage of Maillard reaction.
In the present study, the Nα-acetyl-lysine-glucose model had been successfully set up to produce ARP, Nα-acetyl-lysine-glucose.
After HPLC purification, ARP had been identified by ESI-MS with intense [M+H]+ ion at 351 m/z and the purity of ARP was confirmed to be over 90% by the relative intensity of [M+H]+ ion.
Further structural characterization of the ARP was accomplished by using nuclear magnetic resonance (NMR) spectroscopy, including 1D 1H NMR and 13C NMR, the distortionless enhancement by polarization transfer (DEPT-135) and 2D 1H-1H and 13C-1H correlation spectroscopy (COSY) and 2D nuclear overhauser enhancement spectroscopy (NOESY).
The complexity of 1D 1H NMR and 13C NMR was observed due to the presence of isomers in glucose moiety of ARP.
However, DEPT-135 and 2D NMR techniques provided more structural information to assign the 1H and 13C resonances of ARP.
2D NOESY had successfully confirmed the glycosylated site between 10-N in Nα-acetyl-lysine and 7′-C in glucose.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Chuanjiang& Wang, Hui& Juárez, Manuel& Ruan, Eric Dongliang. 2014. Structural Characterization of Amadori Rearrangement Product of Glucosylated Nα-Acetyl-Lysine by Nuclear Magnetic Resonance Spectroscopy. International Journal of Spectroscopy،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-498181
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Chuanjiang…[et al.]. Structural Characterization of Amadori Rearrangement Product of Glucosylated Nα-Acetyl-Lysine by Nuclear Magnetic Resonance Spectroscopy. International Journal of Spectroscopy No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-498181
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Chuanjiang& Wang, Hui& Juárez, Manuel& Ruan, Eric Dongliang. Structural Characterization of Amadori Rearrangement Product of Glucosylated Nα-Acetyl-Lysine by Nuclear Magnetic Resonance Spectroscopy. International Journal of Spectroscopy. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-498181
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-498181
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر