Isolation and Partial Characterization of Bioactive Fucoxanthin from Himanthalia elongata Brown Seaweed : A TLC-Based Approach
المؤلفون المشاركون
Rajauria, Gaurav
Abu-Ghannam, Nissreen
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-05-09
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Seaweeds are important sources of carotenoids, and numerous studies have shown the beneficial effects of these pigments on human health.
In the present study, Himanthalia elongata brown seaweed was extracted with a mixture of low polarity solvents, and the crude extract was separated using analytical thin-layer chromatography (TLC).
The separated compounds were tested for their potential antioxidant capacity and antimicrobial activity against Listeria monocytogenes bacteria using TLC bioautography approach.
For bio-autography, the coloured band on TLC chromatogram was visualized after spraying with DPPH and triphenyl-tetrazolium chloride reagents which screen antioxidant and antimicrobial compounds, respectively, and only one active compound was screened on the TLC plate.
Preliminary identification of this active compound was done by comparing its colour and Rf (retention factor) value with the authentic fucoxanthin standard.
Further, the active compound was purified using preparative TLC.
This purified compound showed a strong antioxidant (EC50: 14.8±1.27 µg/mL) and antimicrobial (inhibition zone: 10.27 mm, 25 µg compound/disc) activities, which were examined by DPPH scavenging and agar disc-diffusion bioassay, respectively.
The bioactivity shown by the purified compound was almost similar to the fucoxanthin standard.
The characteristic UV-visible and FT-IR spectra of the purified active compound completely matched with the standard.
Hence, the main active compound in H.
elongata was identified as fucoxanthin.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rajauria, Gaurav& Abu-Ghannam, Nissreen. 2013. Isolation and Partial Characterization of Bioactive Fucoxanthin from Himanthalia elongata Brown Seaweed : A TLC-Based Approach. International Journal of Analytical Chemistry،Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-499222
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rajauria, Gaurav& Abu-Ghannam, Nissreen. Isolation and Partial Characterization of Bioactive Fucoxanthin from Himanthalia elongata Brown Seaweed : A TLC-Based Approach. International Journal of Analytical Chemistry No. 2013 (2013), pp.1-6.
https://search.emarefa.net/detail/BIM-499222
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rajauria, Gaurav& Abu-Ghannam, Nissreen. Isolation and Partial Characterization of Bioactive Fucoxanthin from Himanthalia elongata Brown Seaweed : A TLC-Based Approach. International Journal of Analytical Chemistry. 2013. Vol. 2013, no. 2013, pp.1-6.
https://search.emarefa.net/detail/BIM-499222
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-499222
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر