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Subtracted Transcriptome Profile of Tiger Shrimp (Penaeus monodon) That Survived WSSV Challenge
المؤلفون المشاركون
Maralit, Benedict A.
Santos, Mudjekeewis D.
Amar, Edgar C.
Ventolero, Minerva Fatimae H.
Maningas, Mary Beth B.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-08
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الملخص EN
There is increased interest in the development of virus-resistant or improved shrimp stock because production is currently hindered by outbreaks and limited understanding of shrimp defense.
Recent advancement now allows for high-throughput molecular studies on shrimp immunity.
We used next-generation sequencing (NGS) coupled with suppression subtractive hybridization (SSH) to generate a transcriptome database of genes from tiger shrimp that survived White spot syndrome virus (WSSV) challenge.
A total of 9,597 unique sequences were uploaded to NCBI Sequence Read Archive with accession number SRR577080.
Sixty-five unique sequences, 6% of the total, were homologous to genes of Penaeus monodon.
Genes that were initially related to bacterial infection and environmental stress such as 14-3-3 gene, heat shock protein 90, and calreticulin were also found including a few full-length gene sequences.
Initial analysis of the expression of some genes was done.
Hemocyanin, ferritin, and fortilin-binding protein exhibited differential expression between survivor and control tiger shrimps.
Furthermore, candidate microsatellite markers for brood stock selection were mined and tested.
Four trinucleotide and one dinucleotide microsatellites were successfully amplified.
The study highlights the advantage of the NGS platform coupled with SSH in terms of gene discovery and marker generation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Maralit, Benedict A.& Ventolero, Minerva Fatimae H.& Maningas, Mary Beth B.& Amar, Edgar C.& Santos, Mudjekeewis D.. 2014. Subtracted Transcriptome Profile of Tiger Shrimp (Penaeus monodon) That Survived WSSV Challenge. Dataset Papers in Science،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-499687
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Maralit, Benedict A.…[et al.]. Subtracted Transcriptome Profile of Tiger Shrimp (Penaeus monodon) That Survived WSSV Challenge. Dataset Papers in Science No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-499687
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Maralit, Benedict A.& Ventolero, Minerva Fatimae H.& Maningas, Mary Beth B.& Amar, Edgar C.& Santos, Mudjekeewis D.. Subtracted Transcriptome Profile of Tiger Shrimp (Penaeus monodon) That Survived WSSV Challenge. Dataset Papers in Science. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-499687
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-499687
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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