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Age and SPARC Change the Extracellular Matrix Composition of the Left Ventricle
المؤلفون المشاركون
Weintraub, Susan T.
Bradshaw, Amy D.
Baicu, Catalin F.
de Castro Brás, Lisandra E.
Toba, Hiroe
Lindsey, Merry L.
Zile, Michael R.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-24
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Secreted protein acidic and rich in cysteine (SPARC), a collagen-binding matricellular protein, has been implicated in procollagen processing and deposition.
The aim of this study was to investigate age- and SPARC-dependent changes in protein composition of the cardiac extracellular matrix (ECM).
We studied 6 groups of mice (n=4/group): young (4-5 months old), middle-aged (11-12 m.o.), and old (18–29 m.o.) C57BL/6J wild type (WT) and SPARC null.
The left ventricle (LV) was decellularized to enrich for ECM proteins.
Protein extracts were separated by SDS-PAGE, digested in-gel, and analyzed by HPLC-ESI-MS/MS.
Relative quantification was performed by spectral counting, and changes in specific proteins were validated by immunoblotting.
We identified 321 proteins, of which 44 proteins were extracellular proteins.
Of these proteins, collagen III levels were lower in the old null mice compared to WT, suggestive of a role for SPARC in collagen deposition.
Additionally, fibrillin showed a significant increase in the null middle-aged group, suggestive of increased microfibril deposition in the absence of SPARC.
Collagen VI increased with age in both genotypes (>3-fold), while collagen IV showed increased age-associated levels only in the WT animals (4-fold, P<0.05).
These changes may explain the previously reported age-associated increases in LV stiffness.
In summary, our data suggest SPARC is a possible therapeutic target for aging induced LV dysfunction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
de Castro Brás, Lisandra E.& Toba, Hiroe& Baicu, Catalin F.& Zile, Michael R.& Weintraub, Susan T.& Lindsey, Merry L.…[et al.]. 2014. Age and SPARC Change the Extracellular Matrix Composition of the Left Ventricle. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-499921
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
de Castro Brás, Lisandra E.…[et al.]. Age and SPARC Change the Extracellular Matrix Composition of the Left Ventricle. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-499921
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
de Castro Brás, Lisandra E.& Toba, Hiroe& Baicu, Catalin F.& Zile, Michael R.& Weintraub, Susan T.& Lindsey, Merry L.…[et al.]. Age and SPARC Change the Extracellular Matrix Composition of the Left Ventricle. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-499921
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-499921
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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