Fecal Collection and Stabilization Methods for Improved Fecal DNA Test for Colorectal Cancer in a Screening Setting
المؤلفون المشاركون
Carozzi, Francesca Maria
Sani, Cristina
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-11-24
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Early detection of CRC and adenomas reduces CRC-related mortality.
The optimal screening test for CRC is still a subject of debate, and molecular stool sample analysis could provide a valid alternative to conventional methods in terms of compliance and practicability.
Seven fecal DNA storage systems were evaluated in two successive phases.
In the first phase of the study was selected the preservative buffer able to ensure the best human DNA recovery.
In the second phase was evaluated human DNA stability, amplificability and integrity in DNA extracted from selected buffer.
Results showed that the best performance was obtained in samples stored in 100 mM EDTA buffer and Genefec buffer.
Likewise buffer addition yielded a significant increase in DNA stability and integrity without PCR inhibition, compared to the matched aliquots with no buffer added.
Our study shows that samples collected in stabilization solution stabilize DNA so that intact nucleic acids, are more effectively detectable in the molecular assay.
DNA buffer preservation and storage conditions could be useful to guarantee the most consistent yield in human DNA.
Stabilization buffer addition to stool samples prior to transport presents an easily implemented solution that appears to be highly effective.
Overall DNA extracted from faeces preserved in preservative buffer can feasibility been used for molecular analysis leading to an increase of assay sensitivity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Carozzi, Francesca Maria& Sani, Cristina. 2013. Fecal Collection and Stabilization Methods for Improved Fecal DNA Test for Colorectal Cancer in a Screening Setting. Journal of Cancer Research،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-500597
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Carozzi, Francesca Maria& Sani, Cristina. Fecal Collection and Stabilization Methods for Improved Fecal DNA Test for Colorectal Cancer in a Screening Setting. Journal of Cancer Research No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-500597
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Carozzi, Francesca Maria& Sani, Cristina. Fecal Collection and Stabilization Methods for Improved Fecal DNA Test for Colorectal Cancer in a Screening Setting. Journal of Cancer Research. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-500597
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-500597
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر