The Creation of Cybrids Harboring Mitochondrial Haplogroups in the Taiwanese Population of Ethnic Chinese Background : An Extensive In Vitro Tool for the Study of Mitochondrial Genomic Variations
المؤلفون المشاركون
Chen, Shang-Der
Lin, Tsu-Kung
Chuang, Jiin-Haur
Liou, Chia-Wei
Tiao, Mao-Meng
Wang, Pei-Wen
Lin, Hung-Yu
Chen, Jin-Bor
Huang, Sheng-Teng
Chuang, Yao-Chung
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-12-06
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
Mitochondrial DNA (mtDNA) haplogroups may contribute to the development of aging-related diseases.
A reliable in vitro cellular system for investigating the physiologic significance of mtDNA haplogroups is essential.
This study aims to construct and characterize a series of cybrid cell lines harboring variant mtDNA haplogroups collected from healthy Taiwanese volunteers.
Cybrid cells harboring different mtDNA haplogroups like B4a, B4b, B4c, B4d, B5, R, F1a, F2, D4e, D4a, D5b, D5a, E, M8, C, and N9a were prepared.
Luminex 1000 and full-length mtDNA sequencing were used to confirm that mtDNA haplogroups of transmitochondrial cybrids were identical to their original donors.
Cybrid B4b had a significantly lower oxygen consumption rate and higher mitochondrial membrane potential compared to F1a, B5, D5a, D4a, and N9a but had more susceptibility to H2O2-induced oxidative stress than cybrid F1a, D4a, and N9a.
Cybrid N9a had better oxygen consumption and H2O2-challenged viability compared to B4b, F1a, B5, D5a, and D4a.
A series of cybrid cells harboring the main haplogroups of the Taiwanese population with ethnic Chinese background has been developed in vitro.
With this mtDNA haplogroup population, the underlying mechanisms of aging-related diseases may be better understood, and therapeutic interventions can be accelerated.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lin, Tsu-Kung& Lin, Hung-Yu& Chen, Shang-Der& Chuang, Yao-Chung& Chuang, Jiin-Haur& Wang, Pei-Wen…[et al.]. 2012. The Creation of Cybrids Harboring Mitochondrial Haplogroups in the Taiwanese Population of Ethnic Chinese Background : An Extensive In Vitro Tool for the Study of Mitochondrial Genomic Variations. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-501046
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lin, Tsu-Kung…[et al.]. The Creation of Cybrids Harboring Mitochondrial Haplogroups in the Taiwanese Population of Ethnic Chinese Background : An Extensive In Vitro Tool for the Study of Mitochondrial Genomic Variations. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-501046
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lin, Tsu-Kung& Lin, Hung-Yu& Chen, Shang-Der& Chuang, Yao-Chung& Chuang, Jiin-Haur& Wang, Pei-Wen…[et al.]. The Creation of Cybrids Harboring Mitochondrial Haplogroups in the Taiwanese Population of Ethnic Chinese Background : An Extensive In Vitro Tool for the Study of Mitochondrial Genomic Variations. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-501046
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-501046
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر