Comparability of Microarray Data between Amplified and Non Amplified RNA in Colorectal Carcinoma
المؤلفون المشاركون
Papadopoulos, Thomas
Zeittraeger, Isabel
Lausen, Berthold
Schellerer, Vera
Hohenberger, Werner
Dimmler, Arno
Wein, Axel
Brueckl, Wolfgang M.
Schildberg, Claus
Hahn, Eckhart G.
Croner, Roland S.
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-10-11
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Microarray analysis reaches increasing popularity during the investigation of prognostic gene clusters in oncology.
The standardisation of technical procedures will be essential to compare various datasets produced by different research groups.
In several projects the amount of available tissue is limited.
In such cases the preamplification of RNA might be necessary prior to microarray hybridisation.
To evaluate the comparability of microarray results generated either by amplified or non amplified RNA we isolated RNA from colorectal cancer samples (stage UICC IV) following tumour tissue enrichment by macroscopic manual dissection (CMD).
One part of the RNA was directly labelled and hybridised to GeneChips (HG-U133A, Affymetrix), the other part of the RNA was amplified according to the “Eberwine” protocol and was then hybridised to the microarrays.
During unsupervised hierarchical clustering the samples were divided in groups regarding the RNA pre-treatment and 5.726 differentially expressed genes were identified.
Using independent microarray data of 31 amplified vs.
24 non amplified RNA samples from colon carcinomas (stage UICC III) in a set of 50 predictive genes we validated the amplification bias.
In conclusion microarray data resulting from different pre-processing regarding RNA pre-amplification can not be compared within one analysis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Croner, Roland S.& Lausen, Berthold& Schellerer, Vera& Zeittraeger, Isabel& Wein, Axel& Schildberg, Claus…[et al.]. 2009. Comparability of Microarray Data between Amplified and Non Amplified RNA in Colorectal Carcinoma. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-502081
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Croner, Roland S.…[et al.]. Comparability of Microarray Data between Amplified and Non Amplified RNA in Colorectal Carcinoma. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-502081
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Croner, Roland S.& Lausen, Berthold& Schellerer, Vera& Zeittraeger, Isabel& Wein, Axel& Schildberg, Claus…[et al.]. Comparability of Microarray Data between Amplified and Non Amplified RNA in Colorectal Carcinoma. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-502081
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-502081
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر