Impact Assessment of Cadmium Toxicity and Its Bioavailability in Human Cell Lines (Caco-2 and HL-7702)‎

المؤلفون المشاركون

Liu, Di
Rafiq, M. T.
Li, Tingqiang
Lu, Lingli
Daud, M. K.
Yang, Jie
Yang, Xiaoe
He, Zhenli L.
Aziz, Rukhsanda

المصدر

BioMed Research International

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-02-16

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الطب البشري

الملخص EN

Cadmium (Cd) is a widespread environmental toxic contaminant, which causes serious health-related problems.

In this study, human intestinal cell line (Caco-2 cells) and normal human liver cell line (HL-7702 cells) were used to investigate the toxicity and bioavailability of Cd to both cell lines and to validate these cell lines as in vitro models for studying Cd accumulation and toxicity in human intestine and liver.

Results showed that Cd uptake by both cell lines increased in a dose-dependent manner and its uptake by Caco-2 cells (720.15 µg mg−1 cell protein) was significantly higher than HL-7702 cells (229.01 µg mg−1 cell protein) at 10 mg L−1.

A time- and dose-dependent effect of Cd on cytotoxicity assays (LDH release, MTT assay) was observed in both Cd-treated cell lines.

The activities of antioxidant enzymes and differentiation markers (SOD, GPX, and AKP) of the HL-7702 cells were higher than those of Caco-2 cells, although both of them decreased significantly with raising Cd levels.

The results from the present study indicate that Cd above a certain level inhibits cellular antioxidant activities and HL-7702 cells are more sensitive to Cd exposure than Caco-2 cells.

However, Cd concentrations <0.5 mg L−1 pose no toxic effects on both cell lines.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Aziz, Rukhsanda& Rafiq, M. T.& Yang, Jie& Liu, Di& Lu, Lingli& He, Zhenli L.…[et al.]. 2014. Impact Assessment of Cadmium Toxicity and Its Bioavailability in Human Cell Lines (Caco-2 and HL-7702). BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-502310

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Aziz, Rukhsanda…[et al.]. Impact Assessment of Cadmium Toxicity and Its Bioavailability in Human Cell Lines (Caco-2 and HL-7702). BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-502310

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Aziz, Rukhsanda& Rafiq, M. T.& Yang, Jie& Liu, Di& Lu, Lingli& He, Zhenli L.…[et al.]. Impact Assessment of Cadmium Toxicity and Its Bioavailability in Human Cell Lines (Caco-2 and HL-7702). BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-502310

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-502310