Comprehensive Analysis of Bacterial Flora in Postoperative Maxillary Cyst Fluid by 16S rRNA Gene and Culture Methods
المؤلفون المشاركون
Miyamoto, Hiroshi
Taniguchi, Hatsumi
Sano, Naoto
Yamashita, Yoshio
Goto, Masaaki
Fukuda, Kazumasa
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-05-22
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Intracystic fluid was aseptically collected from 11 patients with postoperative maxillary cyst (POMC), and DNA was extracted from the POMC fluid.
Bacterial species were identified by sequencing after cloning of approximately 580 bp of the 16S rRNA gene.
Identification of pathogenic bacteria was also performed by culture methods.
The phylogenetic identity was determined by sequencing 517–596 bp in each of the 1139 16S rRNA gene clones.
A total of 1114 clones were classified while the remaining 25 clones were unclassified.
A total of 103 bacterial species belonging to 42 genera were identified in POMC fluid samples by 16S rRNA gene analysis.
Species of Prevotella (91%), Neisseria (73%), Fusobacterium (73%), Porphyromonas (73%), and Propionibacterium (73%) were found to be highly prevalent in all patients.
Streptococcus mitis (64%), Fusobacterium nucleatum (55%), Propionibacterium acnes (55%), Staphylococcus capitis (55%), and Streptococcus salivarius (55%) were detected in more than 6 of the 11 patients.
The results obtained by the culture method were different from those obtained by 16S rRNA gene analysis, but both approaches may be necessary for the identification of pathogens, especially of bacteria that are difficult to detect by culture methods, and the development of rational treatments for patients with POMC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sano, Naoto& Yamashita, Yoshio& Fukuda, Kazumasa& Taniguchi, Hatsumi& Goto, Masaaki& Miyamoto, Hiroshi. 2012. Comprehensive Analysis of Bacterial Flora in Postoperative Maxillary Cyst Fluid by 16S rRNA Gene and Culture Methods. ISRN Dentistry،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-502398
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sano, Naoto…[et al.]. Comprehensive Analysis of Bacterial Flora in Postoperative Maxillary Cyst Fluid by 16S rRNA Gene and Culture Methods. ISRN Dentistry No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-502398
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sano, Naoto& Yamashita, Yoshio& Fukuda, Kazumasa& Taniguchi, Hatsumi& Goto, Masaaki& Miyamoto, Hiroshi. Comprehensive Analysis of Bacterial Flora in Postoperative Maxillary Cyst Fluid by 16S rRNA Gene and Culture Methods. ISRN Dentistry. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-502398
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-502398
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر