Antimicrobial Activity and Phytochemical Screening of Buchenavia tetraphylla (Aubl.)‎ R. A. Howard (Combretaceae : Combretoideae)‎

الملخص EN

This study evaluated the antimicrobial and hemolytic activities and phytochemical constituents of hydroalcoholic extract and its fractions from Buchenavia tetraphylla leaves.

Cyclohexane (BTCF), ethyl acetate (BTEF), and n-butanol-soluble (BTSBF) and non-soluble (BTNBF) fractions were obtained from a liquid-liquid partition of hydroalcoholic extract (BTHE) from B.

tetraphylla leaves.

The hemolytic activity of active fractions was checked.

The BTHE inhibited the growth of Micrococcus luteus (MIC: 0.10 mg/mL), Pseudomonas aeruginosa (MIC: 0.20 mg/mL), Mycobacterium smegmatis (MIC: 0.39 mg/mL), Proteus vulgaris, and Staphylococcus aureus (MIC: 0.78 mg/mL for both).

The more active fractions were BTCF and BTBSF.

BTCF showed better potential to inhibit M.

luteus (0.10 mg/mL), P.

aeruginosa (0.20 mg/mL), S.

enteritidis (0.39 mg/mL), and S.

aureus (1.56 mg/mL).

BTBSF showed the best results for M.

luteus (0.10 mg/mL), M.

smegmatis, B.

subtilis (0.39 mg/mL for both), and P.

vulgaris (0.10 mg/mL).

The HC50 were greater than observed MIC: 20.30, 4.70 and 2.53 mg/mL, respectively, to BTBF, BTHE and BTCF, which.

The phytochemical analysis detected the presence of flavanoids, triterpene, carbohydrate, and tannin.

Our work showed for the first time the broad-spread antimicrobial activity of B.

tetraphylla, which has nonhemolytic action, creating a new perspective on the interesting association of traditional and scientific knowledge.