Cross-Linked Enzyme Aggregates of Naringinase : Novel Biocatalysts for Naringin Hydrolysis
المؤلفون المشاركون
Ribeiro, Maria H. L.
Rabaça, Marco
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-09-06
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Cross-linked enzyme aggregates (CLEAs) have emerged as interesting biocatalyst design for immobilization.
These new generation enzyme biocatalysts, CLEAs, in addition to exhibiting good mechanical stability, can be highly active, since they do not include large amounts of foreign particulate nonenzymatic material and may have increased stability.
Naringinase (NGase) is an enzyme complex with high potential in pharmaceutical and food industries.
In fact, NGase can be used in the biotransformation of steroids, of antibiotics and mainly on glycosides hydrolysis.
In this paper, the formation of CLEAs was tried using ammonium sulphate, polyethylene glycol 6000 and tert-butyl alcohol as precipitant agents and glutaraldehyde as cross-linking agent, at different pH, time, and temperature conditions.
However, among the precipitant agents tested, only tert-butyl alcohol cross-linked with glutaraldehyde allowed the formation of CLEAs, at pH 4.0 and at temperature between 7 and 10∘C.
Different enzyme loadings were tested.
The NGase-CLEAs were highly effective in naringin hydrolysis.
The operational stability of the NGase-CLEAs aggregates was studied through six successive reutilizations.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ribeiro, Maria H. L.& Rabaça, Marco. 2011. Cross-Linked Enzyme Aggregates of Naringinase : Novel Biocatalysts for Naringin Hydrolysis. Enzyme Research،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-503288
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ribeiro, Maria H. L.& Rabaça, Marco. Cross-Linked Enzyme Aggregates of Naringinase : Novel Biocatalysts for Naringin Hydrolysis. Enzyme Research No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-503288
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ribeiro, Maria H. L.& Rabaça, Marco. Cross-Linked Enzyme Aggregates of Naringinase : Novel Biocatalysts for Naringin Hydrolysis. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-503288
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-503288
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر