A Stability Indicating UPLC Method for the Determination of Tramadol Hydrochloride : Application to Pharmaceutical Analysis
المؤلفون المشاركون
Revanasiddappa, Hosakere Doddarevanna
Raghu, Madihalli Srinivas
Ramesh, Pavagada J.
Vinay, Kanakapura B.
Xavier, Cijo M.
المصدر
Chromatography Research International
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-05-29
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The use of Ultra Performance Liquid Chromatography (UPLC), with a rapid 5-minute reversed phase isocratic separation on a 1.7 μm reversed-phase packing material to provide rapid ‘‘high throughput’’ support for tramadol hydrochloride (TMH) is demonstrated.
A simple, precise and accurate stability-indicating isocratic UPLC method was developed for the determination of TMH in bulk drug and in its tablets.
The method was developed using Waters Aquity BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with mobile phase consisting of a mixture of potassium dihydrogen phosphate buffer of pH 2.8 and an equal volume of acetonitrile (60 : 40 v/v).
The eluted compound was detected at 226 nm with a UV detector.
The standard curve of mean peak area versus concentration showed an excellent linearity over a concentration range 0.5–300 μg mL−1 TMH with regression coefficient (r) value of 0.9999.
The limit of detection (S/N =3) was 0.08 μg mL−1 and the limit of quantification (S/N =10) was 0.2 μg mL−1.
Forced degradation of the bulk sample was conducted an accordance with the ICH guidelines.
Acidic, basic, hydrolytic, oxidative, thermal and photolytic degradation were used to assess the stability indicating power of the method.
TMH was found to degrade significantly in acidic, basic and oxidative stress conditions and stable in thermal, hydrolytic and photolytic conditions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Vinay, Kanakapura B.& Revanasiddappa, Hosakere Doddarevanna& Xavier, Cijo M.& Ramesh, Pavagada J.& Raghu, Madihalli Srinivas. 2012. A Stability Indicating UPLC Method for the Determination of Tramadol Hydrochloride : Application to Pharmaceutical Analysis. Chromatography Research International،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-504972
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Vinay, Kanakapura B.…[et al.]. A Stability Indicating UPLC Method for the Determination of Tramadol Hydrochloride : Application to Pharmaceutical Analysis. Chromatography Research International No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-504972
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Vinay, Kanakapura B.& Revanasiddappa, Hosakere Doddarevanna& Xavier, Cijo M.& Ramesh, Pavagada J.& Raghu, Madihalli Srinivas. A Stability Indicating UPLC Method for the Determination of Tramadol Hydrochloride : Application to Pharmaceutical Analysis. Chromatography Research International. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-504972
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-504972
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر