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Melatonin Suppresses the Expression of 45S Preribosomal RNA and Upstream Binding Factor and Enhances the Antitumor Activity of Puromycin in MDA-MB-231 Breast Cancer Cells
المؤلفون المشاركون
Jung, Ji Hoon
Sohn, Eun Jung
Shin, Eun Ah
Kim, Bonglee
Kim, Ji-Hyun
Park, Yong Koo
Lee, Duckgue
Yun, Miyong
Lee, Hyo-Jeong
Kim, Sung-Hoon
Jung, Deok-Beom
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-04-07
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Since the dysregulation of ribosome biogenesis is closely associated with tumor progression, in the current study, the critical role of ribosome biogenesis related signaling was investigated in melatonin and/or puromycin induced apoptosis in MDA-MB-231 breast cancer cells.
Despite its weak cytotoxicity, melatonin from 3 mM attenuated the expression of 45S pre-ribosomal RNA (pre-rRNA), UBF as a nucleolar transcription factor, and fibrillarin at mRNA level and consistently downregulated nucleolar proteins such as UBF and fibrillarin at protein level in MDA-MB-231 cells.
Furthermore, immunofluorescence assay revealed that UBF was also degraded by melatonin in MDA-MB-231 cells.
In contrast, melatonin attenuated the expression of survival genes such as Bcl-xL, Mcl-1, cyclinD1, and cyclin E, suppressed the phosphorylation of AKT, mTOR, and STAT3, and cleaved PARP and activated caspase 3 only at a high concentration of 12 mM.
However, combined treatment of melatonin (3 mM) and puromycin (1 μM) synergistically inhibited viability, attenuated the expression of 45S pre-rRNA and UBF, and consistently downregulated UBF, XPO1 and IPO7, procaspase 3, and Bcl-xL in MDA-MB 231 cells.
Overall, these findings suggest that melatonin can be a cancer preventive agent by combination with puromycin via the inhibition of 45S pre-rRNA and UBF in MDA-MB 231 breast cancer cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jung, Ji Hoon& Sohn, Eun Jung& Shin, Eun Ah& Lee, Duckgue& Kim, Bonglee& Jung, Deok-Beom…[et al.]. 2013. Melatonin Suppresses the Expression of 45S Preribosomal RNA and Upstream Binding Factor and Enhances the Antitumor Activity of Puromycin in MDA-MB-231 Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-505676
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jung, Ji Hoon…[et al.]. Melatonin Suppresses the Expression of 45S Preribosomal RNA and Upstream Binding Factor and Enhances the Antitumor Activity of Puromycin in MDA-MB-231 Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-505676
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jung, Ji Hoon& Sohn, Eun Jung& Shin, Eun Ah& Lee, Duckgue& Kim, Bonglee& Jung, Deok-Beom…[et al.]. Melatonin Suppresses the Expression of 45S Preribosomal RNA and Upstream Binding Factor and Enhances the Antitumor Activity of Puromycin in MDA-MB-231 Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-505676
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-505676
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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