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Atypical Enteropathogenic Escherichia coli Secretes Plasmid Encoded Toxin
المؤلفون المشاركون
Rossato, Sarita S.
Piazza, Roxane M. F.
Elias, Waldir P.
Barbosa, Camila M.
Ruiz, Rita C.
Corrêa, Lívia M.
Melo, Keyde C. M.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-11
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Plasmid encoded toxin (Pet) is a serine protease originally described in enteroaggregative Escherichia coli (EAEC) prototype strain 042 whose entire characterization was essentially obtained from studies performed with the purified toxin.
Here we show that Pet is not exclusive to EAEC.
Atypical enteropathogenic Escherichia coli (aEPEC) strains, isolated from diarrhea cases, express Pet and its detection in supernatants of infected HEp-2 cells coincides with the appearance of cell damage, which, in turn, were similar to those described with purified Pet.
Pet secretion and the cytotoxic effects are time and culture medium dependent.
In presence of DMEM supplemented with tryptone cell rounding and detachment were observed after just 5 h of incubation with the bacteria.
In the absence of tryptone, the cytotoxic effects were detected only after 24 h of infection.
We also show that, in addition to the prototype EAEC, other pet+ EAEC strains, also isolated from diarrhea cases, induce cellular damage in the same degree as the aEPEC.
The cytotoxic effects of EAEC and aEPEC strains were significantly reduced in the presence of a serine protease inhibitor or anti-Pet IgG serum.
Our results show a common aspect between the aEPEC and EAEC and provide the first evidence pointing to a role of Pet in aEPEC pathogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ruiz, Rita C.& Melo, Keyde C. M.& Rossato, Sarita S.& Barbosa, Camila M.& Corrêa, Lívia M.& Elias, Waldir P.…[et al.]. 2014. Atypical Enteropathogenic Escherichia coli Secretes Plasmid Encoded Toxin. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-506254
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ruiz, Rita C.…[et al.]. Atypical Enteropathogenic Escherichia coli Secretes Plasmid Encoded Toxin. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-506254
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ruiz, Rita C.& Melo, Keyde C. M.& Rossato, Sarita S.& Barbosa, Camila M.& Corrêa, Lívia M.& Elias, Waldir P.…[et al.]. Atypical Enteropathogenic Escherichia coli Secretes Plasmid Encoded Toxin. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-506254
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-506254
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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