An Attenuated Cytomegalovirus Vaccine with a Deletion of a Viral Chemokine Gene Is Protective against Congenital CMV Transmission in a Guinea Pig Model
المؤلفون المشاركون
Choi, K. Yeon
Schleiss, Mark R.
Lacayo, Juan C.
Wey, Andrew
Leviton, Michael P.
Hernandez-Alvarado, Nelmary
المصدر
Clinical and Developmental Immunology
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-08-20
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Development of a vaccine against congenital cytomegalovirus (CMV) infection is a public health priority, but CMVs encode immune evasion genes that complicate live virus vaccine design.
To resolve this problem, this study employed guanosyl phosphoribosyl transferase (gpt) mutagenesis to generate a recombinant guinea pig CMV (GPCMV) with a knockout of a viral chemokine gene, GPCMV MIP (gp1).
MIP deletion virus replicated with wild-type kinetics in cell culture but was attenuated in nonpregnant guinea pigs, demonstrating reduced viremia and reduced inflammation and histopathology (compared to a control virus with an intact GPCMV MIP gene) following footpad inoculation.
In spite of attenuation, the vaccine was immunogenic, eliciting antibody responses comparable to those observed in natural infection.
To assess its protective potential as a vaccine, either recombinant virus or placebo was used to immunize seronegative female guinea pigs.
Dams were challenged in the early 3rd trimester with salivary gland-adapted GPCMV.
Immunization protected against DNAemia (1/15 in vaccine group versus 12/13 in the control group, P<0.01).
Mean birth weights were significantly higher in pups born to vaccinated dams compared to controls (98.7 g versus 71.2 g, P<0.01).
Vaccination reduced pup mortality, from 35/50 (70%) in controls to 8/52 (15%) in the immunization group.
Congenital GPCMV infection was also reduced, from 35/50 (70%) in controls to 9/52 (17%) in the vaccine group (P<0.0001).
We conclude that deletion of an immune modulation gene can attenuate the pathogenicity of GPCMV while resulting in a viral vaccine that retains immunogenicity and demonstrates efficacy against congenital infection and disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Leviton, Michael P.& Lacayo, Juan C.& Choi, K. Yeon& Hernandez-Alvarado, Nelmary& Wey, Andrew& Schleiss, Mark R.. 2013. An Attenuated Cytomegalovirus Vaccine with a Deletion of a Viral Chemokine Gene Is Protective against Congenital CMV Transmission in a Guinea Pig Model. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-507070
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Leviton, Michael P.…[et al.]. An Attenuated Cytomegalovirus Vaccine with a Deletion of a Viral Chemokine Gene Is Protective against Congenital CMV Transmission in a Guinea Pig Model. Clinical and Developmental Immunology No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-507070
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Leviton, Michael P.& Lacayo, Juan C.& Choi, K. Yeon& Hernandez-Alvarado, Nelmary& Wey, Andrew& Schleiss, Mark R.. An Attenuated Cytomegalovirus Vaccine with a Deletion of a Viral Chemokine Gene Is Protective against Congenital CMV Transmission in a Guinea Pig Model. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-507070
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-507070
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر