Induction by TNF-α of IL-6 and IL-8 in Cystic Fibrosis Bronchial IB3-1 Epithelial Cells Encapsulated in Alginate Microbeads
المؤلفون المشاركون
Breveglieri, Giulia
Nastruzzi, Claudio
Borgatti, Monica
Gambari, Roberto
Mazzitelli, Stefania
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-09-08
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
We have developed a microencapsulation procedure for the entrapment and manipulation of IB3-1 cystic fibrosis cells.
The applied method is based on generation of monodisperse droplets by a vibrational nozzle.
Different experimental parameters were analyzed, including frequency and amplitude of vibration, polymer pumping rate and distance between the nozzle and the gelling bath.
We have found that the microencapsulation procedure does not alter the viability of the encapsulated IB3-1 cells.
The encapsulated IB3-1 cells were characterized in term of secretomic profile, analyzing the culture medium by Bio-Plex strategy.
The experiments demonstrated that most of the analyzed proteins, were secreted both by the free and encapsulated cells, even if in a different extent.
In order to determine the biotechnological applications of this procedure, we determined whether encapsulated IB3-1 cells could be induced to pro-inflammatory responses, after treatment with TNF-α.
In this experimental set-up, encapsulated and free IB3-1 cells were treated with TNF-α, thereafter the culture media from both cell populations were collected.
As expected, TNF-α induced a sharp increase in the secretion of interleukins, chemokines and growth factors.
Of great interest was the evidence that induction of interleukin-6 and interleukin-8 occurs also by encapsulated IB3-1 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Borgatti, Monica& Mazzitelli, Stefania& Breveglieri, Giulia& Gambari, Roberto& Nastruzzi, Claudio. 2010. Induction by TNF-α of IL-6 and IL-8 in Cystic Fibrosis Bronchial IB3-1 Epithelial Cells Encapsulated in Alginate Microbeads. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-507177
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Borgatti, Monica…[et al.]. Induction by TNF-α of IL-6 and IL-8 in Cystic Fibrosis Bronchial IB3-1 Epithelial Cells Encapsulated in Alginate Microbeads. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-11.
https://search.emarefa.net/detail/BIM-507177
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Borgatti, Monica& Mazzitelli, Stefania& Breveglieri, Giulia& Gambari, Roberto& Nastruzzi, Claudio. Induction by TNF-α of IL-6 and IL-8 in Cystic Fibrosis Bronchial IB3-1 Epithelial Cells Encapsulated in Alginate Microbeads. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-11.
https://search.emarefa.net/detail/BIM-507177
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-507177
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر