The Effects of Substituting Glassware for Plasticware and the Use of an Ethanol Vector on Oocyte Maturation In Vitro
المؤلفون المشاركون
Hamilton, C. K.
Bartlewski, P. M.
King, W. A.
Macaulay, A. D.
المصدر
Veterinary Medicine International
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-03-14
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The intent of this study was to evaluate specific technical aspects of in vitro oocyte maturation (IVM), which included container material and solvent delivery vector.
Oocytes were matured in oil-free, open-well systems contained in either plastic or glass dishes and compared to control oocytes matured in media droplets on plastic dishes overlaid with mineral oil.
Open-well experiments were repeated with ethanol in a quantity sufficient for delivery of nonmiscible compounds.
Cleavage rates were significantly decreased in the glassware system when compared to controls.
The plasticware open-well system did not differ from either the controls or the glassware groups.
Cleavage in glassware with ethanol was significantly lower than controls or plasticware with ethanol.
Blastocyst rates were only decreased in the glassware-ethanol treatment when compared to plasticware-ethanol treatment.
Cell counts and percentage of TUNEL-positive cells did not differ significantly.
Unexpectedly, sex ratio was significantly decreased (34% male) from the expected value of 50% male in the glassware group with added ethanol.
The current study demonstrates the sensitivity of IVM to subtle technical changes, resulting in significant developmental consequences.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Macaulay, A. D.& Hamilton, C. K.& Bartlewski, P. M.& King, W. A.. 2012. The Effects of Substituting Glassware for Plasticware and the Use of an Ethanol Vector on Oocyte Maturation In Vitro. Veterinary Medicine International،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-507699
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Macaulay, A. D.…[et al.]. The Effects of Substituting Glassware for Plasticware and the Use of an Ethanol Vector on Oocyte Maturation In Vitro. Veterinary Medicine International No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-507699
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Macaulay, A. D.& Hamilton, C. K.& Bartlewski, P. M.& King, W. A.. The Effects of Substituting Glassware for Plasticware and the Use of an Ethanol Vector on Oocyte Maturation In Vitro. Veterinary Medicine International. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-507699
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-507699
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر