Electroacupuncture Could Regulate the NF-κB Signaling Pathway to Ameliorate the Inflammatory Injury in Focal Cerebral IschemiaReperfusion Model Rats

الملخص EN

The activated nuclear factor-KappaB signaling pathway plays a critical role in inducing inflammatory injury.

It has been reported that electroacupuncture could be an effective anti-inflammatory treatment.

We aimed to explore the complex mechanism by which EA inhibits the activation of the NF-κB signal pathway and ameliorate inflammatory injury in the short term; the effects of NEMO Binding Domain peptide for this purpose were compared.

Focal cerebral I/R was induced by middle cerebral artery occlusion for 2 hrs.

Total 380 male Sprague-Dawley rats are in the study.

The neurobehavioral scores, infarction volumes, and the levels of IL-1β and IL-13 were detected.

NF-κB p65, IκBα, IKKα, and IKKβ were analyzed and the ability of NF-κB binding DNA was investigated.

The EA treatment and the NBD peptide treatment both reduced infarct size, improved neurological scores, and regulated the levels of IL-1β and IL-13.

The treatment reduced the expression of IKKα and IKKβ and altered the expression of NF-κB p65 and IκBα in the cytoplasm and nucleus; the activity of NF-κB was effectively reduced.

We conclude that EA treatment might interfere with the process of NF-κB nuclear translocation.

And it also could suppress the activity of NF-κB signaling pathway to ameliorate the inflammatory injury after focal cerebral ischemia/reperfusion.