Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro

المؤلفون المشاركون

Tarasov, Sergey A.
Epstein, Oleg I.
Nicoll, Jim
Gorbunov, Evgeniy A.

المصدر

International Journal of Endocrinology

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-4، 4ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-04-18

دولة النشر

مصر

عدد الصفحات

4

التخصصات الرئيسية

الأحياء

الملخص EN

Purpose.

To investigate the mechanism of action in peripheral tissues of novel complex drug containing release-active dilutions of antibodies to the beta subunit of the insulin receptor and antibodies to endothelial nitric oxide synthase (Subetta), which has shown efficacy in animal models of diabetes.

Methods.

Human mature adipocytes were incubated either with Subetta, with one of negative controls (placebo or vehicle), with one of nonspecific controls (release-active dilutions of antibodies to cannabinoid receptor type I or release-active dilutions of rabbit nonimmune serum), or with dimethyl sulfoxide (DMSO) at 37°C in a humidified incubator at 5% CO2 for three days.

Rosiglitazone was used as reference drug.

Secretion of adiponectin was measured by quantitative enzyme-linked immunosorbent assay (ELISA).

Results.

Only Subetta significantly stimulates adiponectin production by mature human adipocytes.

Nonspecific controls did not significantly affect adiponectin secretion, resulting in adiponectin levels comparable to background values of the negative controls and DMSO.

Conclusion.

Increasing adiponectin production in absence of insulin by Subetta probably via modulating effect on the beta subunit of the insulin receptor might serve as one of the mechanisms of the antidiabetic effect of this drug.

These in vitro results give first insight on possible mechanism of action of Subetta and serve as a background for further studies.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Nicoll, Jim& Gorbunov, Evgeniy A.& Tarasov, Sergey A.& Epstein, Oleg I.. 2013. Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro. International Journal of Endocrinology،Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-508626

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Nicoll, Jim…[et al.]. Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro. International Journal of Endocrinology No. 2013 (2013), pp.1-4.
https://search.emarefa.net/detail/BIM-508626

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Nicoll, Jim& Gorbunov, Evgeniy A.& Tarasov, Sergey A.& Epstein, Oleg I.. Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro. International Journal of Endocrinology. 2013. Vol. 2013, no. 2013, pp.1-4.
https://search.emarefa.net/detail/BIM-508626

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-508626