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Impact of Trichloroethylene Exposure on the Microbial Diversity and Protein Expression in Anaerobic Granular Biomass at 37°C and 15°C
المؤلفون المشاركون
O'Flaherty, Vincent
Enright, Anne-Marie
Botting, Catherine
Abram, Florence
Siggins, Alma
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-08
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Granular biomass from a laboratory-scale anaerobic bioreactor trial was analysed to identify changes in microbial community structure and function in response to temperature and trichloroethylene (TCE).
Two bioreactors were operated at 37°C, while two were operated at 15°C.
At the time of sampling, one of each temperature pair of bioreactors was exposed to process failure-inducing concentrations of TCE (60 mg L−1) while the other served as a TCE-free control.
Bacterial community structure was investigated using denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene clone library analysis.
Temperature was identified as an important factor for bacterial community composition, while minor differences were associated with trichloroethylene supplementation.
Proteobacteria was the dominant phylum in all bioreactors, while clone library analysis revealed a higher proportion of Bacteroidetes-, Chloroflexi-, and Firmicutes-like clones at 15°C than at 37°C.
Comparative metaproteomics in the presence and absence of TCE was carried out by two-dimensional gel electrophoresis (2-DGE), and 28 protein spots were identified, with putative functions related to cellular processes, including methanogenesis, glycolysis, the glyoxylate cycle, and the methyl malonyl pathway.
A good agreement between metaproteomic species assignment and phylogenetic information was observed, with 10 of the identified proteins associated with members of the phylum Proteobacteria.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Siggins, Alma& Enright, Anne-Marie& Abram, Florence& Botting, Catherine& O'Flaherty, Vincent. 2012. Impact of Trichloroethylene Exposure on the Microbial Diversity and Protein Expression in Anaerobic Granular Biomass at 37°C and 15°C. Archaea،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-509885
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Siggins, Alma…[et al.]. Impact of Trichloroethylene Exposure on the Microbial Diversity and Protein Expression in Anaerobic Granular Biomass at 37°C and 15°C. Archaea No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-509885
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Siggins, Alma& Enright, Anne-Marie& Abram, Florence& Botting, Catherine& O'Flaherty, Vincent. Impact of Trichloroethylene Exposure on the Microbial Diversity and Protein Expression in Anaerobic Granular Biomass at 37°C and 15°C. Archaea. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-509885
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-509885
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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