Immobilization of Murine Anti-BMP-2 Monoclonal Antibody on Various Biomaterials for Bone Tissue Engineering
المؤلفون المشاركون
Zadeh, Homayoun H.
Almohaimeed, Mohammad
Freire, Marcelo O.
Abdelhamid, Alaa I.
Ansari, Sahar
Pang, Eun-Kyoung
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-23
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Biomaterials are widely used as scaffolds for tissue engineering.
We have developed a strategy for bone tissue engineering that entails application of immobilized anti-BMP-2 monoclonal antibodies (mAbs) to capture endogenous BMPs in vivo and promote antibody-mediated osseous regeneration (AMOR).
The purpose of the current study was to compare the efficacy of immobilization of a specific murine anti-BMP-2 mAb on three different types of biomaterials and to evaluate their suitability as scaffolds for AMOR.
Anti-BMP-2 mAb or isotype control mAb was immobilized on titanium (Ti) microbeads, alginate hydrogel, and ACS.
The treated biomaterials were surgically implanted in rat critical-sized calvarial defects.
After 8 weeks, de novo bone formation was assessed using micro-CT and histomorphometric analyses.
Results showed de novo bone regeneration with all three scaffolds with immobilized anti-BMP-2 mAb, but not isotype control mAb.
Ti microbeads showed the highest volume of bone regeneration, followed by ACS.
Alginate showed the lowest volume of bone.
Localization of BMP-2, -4, and -7 antigens was detected on all 3 scaffolds with immobilized anti-BMP-2 mAb implanted in calvarial defects.
Altogether, these data suggested a potential mechanism for bone regeneration through entrapment of endogenous BMP-2, -4, and -7 proteins leading to bone formation using different types of scaffolds via AMOR.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ansari, Sahar& Freire, Marcelo O.& Pang, Eun-Kyoung& Abdelhamid, Alaa I.& Almohaimeed, Mohammad& Zadeh, Homayoun H.. 2014. Immobilization of Murine Anti-BMP-2 Monoclonal Antibody on Various Biomaterials for Bone Tissue Engineering. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-509959
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ansari, Sahar…[et al.]. Immobilization of Murine Anti-BMP-2 Monoclonal Antibody on Various Biomaterials for Bone Tissue Engineering. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-509959
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ansari, Sahar& Freire, Marcelo O.& Pang, Eun-Kyoung& Abdelhamid, Alaa I.& Almohaimeed, Mohammad& Zadeh, Homayoun H.. Immobilization of Murine Anti-BMP-2 Monoclonal Antibody on Various Biomaterials for Bone Tissue Engineering. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-509959
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-509959
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر