Anti-Inflammatory Effect and Mechanism of the Green Fruit Extract of Solanum integrifolium Poir.

الملخص EN

The green fruit of Solanum integrifolium Poir.

has been used traditionally as an anti-inflammatory and analgesic remedy in Taiwanese aboriginal medicine.

The goal of this study is to evaluate the anti-inflammatory activity and mechanism of the green fruit extract of S.

integrifolium.

A bioactivity-guided fractionation procedure was developed to identify the active partition fraction.

The methanol fraction (ME), with the highest phenolic content, exhibited the strongest inhibitory effect against LPS-mediated nitric oxide (NO) release and cytotoxicity in RAW264.7 macrophages.

ME also significantly downregulated the expression of LPS-induced proinflammatory genes, such as iNOS, COX-2, IL-1β, IL-6, CCL2/MCP-1, and CCL3/MIP1α.

Moreover, ME significantly upregulated HO-1 expression and stimulated the activation of extracellular-signal-regulated kinase 1/2 (ERK1/2).

Pretreatment of cells with the HO-1 inhibitor zinc protoporphyrin and MEK/ERK inhibitor U0126 attenuated ME’s inhibitory activity against LPS-induced NO production.

Taken together, this is the first study to demonstrate the anti-inflammatory activity of green fruit extract of S.

integrifolium and its activity may be mediated by the upregulation of HO-1 expression and activation of ERK1/2 pathway.