Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of β-Adrenergic Receptors to Phosphoinositide 3-Kinase
المصدر
International Journal of Cell Biology
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-02-05
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
An agonist-occupied β2-adrenergic receptor (β2-AR) recruits G protein receptor kinase-2 (GRK2) which is recruited to the membrane.
Thus, the physical proximity of activated β2-AR and PI-3K allows the activation of the latter.
In contrast, it has been observed that the β1-AR is unable to activate the PI-3K/Akt pathway.
We hypothesized that the difference might be due to molecular determinants present in the carboxy termini of the two β-AR subtypes.
Using transiently transfected HEK 293 cells expressing either β1- or β2-AR, we also observed that in presence of an agonist, β2-AR, but not β1-AR, is able to activate the PI-3K/Akt pathway.
Switching the seventh transmembrane domain and the carboxy tail between the two receptors reverses this phenotype; that is, β1×β2-AR can activate the PI-3K/Akt pathway whereas β2×β1-AR cannot.
Pretreatment with pertussis toxin abolished the activation of PI-3K by β2- or β1×β2-AR stimulation.
Ligand-mediated internalization of the β2-AR induced by a 15-minute stimulation with agonist was abolished in the presence of a dominant negative of PI-3K or following pertussis toxin pretreatment.
These results indicate that the subtype-specific differences in the coupling to PI-3K/Akt pathway are due to molecular determinants present in the carboxy tail of the receptor and further that β2-AR activates PI-3K via a pertussis toxin-sensitive mechanism.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
2009. Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of β-Adrenergic Receptors to Phosphoinositide 3-Kinase. International Journal of Cell Biology،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-511499
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of β-Adrenergic Receptors to Phosphoinositide 3-Kinase. International Journal of Cell Biology No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-511499
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of β-Adrenergic Receptors to Phosphoinositide 3-Kinase. International Journal of Cell Biology. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-511499
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-511499
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر