Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells
المؤلفون المشاركون
Seigel, Gail M.
Cassidy, Linda
Chang, Rui
Meyer, Jason
Choi, Meerim
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-05-27
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Pluripotent stem cell markers can be useful for diagnostic evaluation of human tumors.
The novel pluripotent marker stage-specific embryonic antigen-5 (SSEA-5) is expressed in undifferentiated human induced pluripotent cells (iPSCs), but little is known about SSEA-5 expression in other primitive tissues (e.g., human tumors).
We evaluated SSEA-5 immunoreactivity patterns in human tumors, cell lines, teratomas, and iPS cells together with another pluripotent cell surface marker L1 cell adhesion molecule (L1CAM).
We tested two hypotheses: (1) SSEA-5 and L1CAM would be immunoreactive and colocalized in human tumors; (2) SSEA-5 and L1CAM immunoreactivity would persist in iPSCs following retinal differentiating treatment.
SSEA-5 immunofluorescence was most pronounced in primitive tumors, such as embryonal carcinoma.
In tumor cell lines, SSEA-5 was highly immunoreactive in Capan-1 cells, while L1CAM was highly immunoreactive in U87MG cells.
SSEA-5 and L1CAM showed colocalization in undifferentiated iPSCs, with immunopositive iPSCs remaining after 20 days of retinal differentiating treatment.
This is the first demonstration of SSEA-5 immunoreactivity in human tumors and the first indication of SSEA-5 and L1CAM colocalization.
SSEA-5 and L1CAM warrant further investigation as potentially useful tumor markers for histological evaluation or as markers to monitor the presence of undifferentiated cells in iPSC populations prior to therapeutic use.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cassidy, Linda& Choi, Meerim& Meyer, Jason& Chang, Rui& Seigel, Gail M.. 2013. Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells. Journal of Biomarkers،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-511629
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cassidy, Linda…[et al.]. Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells. Journal of Biomarkers No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-511629
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cassidy, Linda& Choi, Meerim& Meyer, Jason& Chang, Rui& Seigel, Gail M.. Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells. Journal of Biomarkers. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-511629
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-511629
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر