A Sphingosine Kinase Form 2 Knockout Sensitizes Mouse Myocardium to IschemiaReoxygenation Injury and Diminishes Responsiveness to Ischemic Preconditioning
المؤلفون المشاركون
Li, Luyi
Kelley, Michael
Zhang, Jianqing
Jin, Zhu-Qiu
Honbo, Norman
Karliner, Joel S.
Vessey, Donald A.
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-04-18
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
Sphingosine kinase (SphK) exhibits two isoforms, SphK1 and SphK2.
Both forms catalyze the synthesis of sphingosine 1-phosphate (S1P), a sphingolipid involved in ischemic preconditioning (IPC).
Since the ratio of SphK1 : SphK2 changes dramatically with aging, it is important to assess the role of SphK2 in IR injury and IPC.
Langendorff mouse hearts were subjected to IR (30 min equilibration, 50 min global ischemia, and 40 min reperfusion).
IPC consisted of 2 min of ischemia and 2 min of reperfusion for two cycles.
At baseline, there were no differences in left ventricular developed pressure (LVDP), ± dP/dtmax, and heart rate between SphK2 null (KO) and wild-type (WT) hearts.
In KO hearts, SphK2 activity was undetectable, and SphK1 activity was unchanged compared to WT.
Total SphK activity was reduced by 53%.
SphK2 KO hearts subjected to IR exhibited significantly more cardiac damage (37±1% infarct size) compared with WT (28±1% infarct size); postischemic recovery of LVDP was lower in KO hearts.
IPC exerted cardioprotection in WT hearts.
The protective effect of IPC against IR was diminished in KO hearts which had much higher infarction sizes (35±2%) compared to the IPC/IR group in control hearts (12±1%).
Western analysis revealed that KO hearts had substantial levels of phosphorylated p38 which could predispose the heart to IR injury.
Thus, deletion of the SphK2 gene sensitizes the myocardium to IR injury and diminishes the protective effect of IPC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Vessey, Donald A.& Li, Luyi& Jin, Zhu-Qiu& Kelley, Michael& Honbo, Norman& Zhang, Jianqing…[et al.]. 2011. A Sphingosine Kinase Form 2 Knockout Sensitizes Mouse Myocardium to IschemiaReoxygenation Injury and Diminishes Responsiveness to Ischemic Preconditioning. Oxidative Medicine and Cellular Longevity،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-511649
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Vessey, Donald A.…[et al.]. A Sphingosine Kinase Form 2 Knockout Sensitizes Mouse Myocardium to IschemiaReoxygenation Injury and Diminishes Responsiveness to Ischemic Preconditioning. Oxidative Medicine and Cellular Longevity No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-511649
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Vessey, Donald A.& Li, Luyi& Jin, Zhu-Qiu& Kelley, Michael& Honbo, Norman& Zhang, Jianqing…[et al.]. A Sphingosine Kinase Form 2 Knockout Sensitizes Mouse Myocardium to IschemiaReoxygenation Injury and Diminishes Responsiveness to Ischemic Preconditioning. Oxidative Medicine and Cellular Longevity. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-511649
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-511649
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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