Reversal of Ampicillin Resistance in MRSA via Inhibition of Penicillin-Binding Protein 2a by Acalypha wilkesiana
المؤلفون المشاركون
Ting, Kang Nee
Loh, Hwei-San
Lim, Kuan-Hon
Santiago, Carolina
Pang, Ee Leen
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-30
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
The inhibitory activity of a semipure fraction from the plant, Acalypha wilkesiana assigned as 9EA-FC-B, alone and in combination with ampicillin, was studied against methicillin-resistant Staphylococcus aureus (MRSA).
In addition, effects of the combination treatment on PBP2a expression were investigated.
Microdilution assay was used to determine the minimal inhibitory concentrations (MIC).
Synergistic effects of 9EA-FC-B with ampicillin were determined using the fractional inhibitory concentration (FIC) index and kinetic growth curve assay.
Western blot experiments were carried out to study the PBP2a expression in treated MRSA cultures.
The results showed a synergistic effect between ampicillin and 9EA-FC-B treatment with the lowest FIC index of 0.19 (synergism ≤ 0.5).
The presence of 9EA-FC-B reduced the MIC of ampicillin from 50 to 1.56 μg mL−1.
When ampicillin and 9EA-FC-B were combined at subinhibitory level, the kinetic growth curves were suppressed.
The antibacterial effect of 9EA-FC-B and ampicillin was shown to be synergistic.
The synergism is due the ability of 9EA-FC-B to suppress the activity of PBP2a, thus restoring the susceptibility of MRSA to ampicillin.
Corilagin was postulated to be the constituent responsible for the synergistic activity showed by 9EA-FC-B.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Santiago, Carolina& Pang, Ee Leen& Lim, Kuan-Hon& Loh, Hwei-San& Ting, Kang Nee. 2014. Reversal of Ampicillin Resistance in MRSA via Inhibition of Penicillin-Binding Protein 2a by Acalypha wilkesiana. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-512037
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Santiago, Carolina…[et al.]. Reversal of Ampicillin Resistance in MRSA via Inhibition of Penicillin-Binding Protein 2a by Acalypha wilkesiana. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-512037
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Santiago, Carolina& Pang, Ee Leen& Lim, Kuan-Hon& Loh, Hwei-San& Ting, Kang Nee. Reversal of Ampicillin Resistance in MRSA via Inhibition of Penicillin-Binding Protein 2a by Acalypha wilkesiana. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-512037
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-512037
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر