Integration of Kinetic Analysis of Reaction Curve with a Proper Classical Approach for Enzymatic Analysis
المؤلفون المشاركون
Yang, Xiaolan
Liao, Fei
Long, Gaobo
Liao, Pu
Jiang, Hairong
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-05-03
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الطب البشري
تكنولوجيا المعلومات وعلم الحاسوب
الملخص EN
For enzymatic analysis to quantify a substrate or enzyme, kinetic analysis of reaction curve can be integrated with a proper classical approach.
For their integration, they should have consistent slopes and intercepts of linear response and an overlapped region of analyte quantities measurable under optimized conditions.
To quantify a substrate after optimizations of tool enzyme activity and reaction duration, the equilibrium method works when the reaction is completed within the reaction duration; otherwise, kinetic analysis of reaction curve applies providing at least seven data with sufficient consumption of substrate.
To quantify an enzyme after optimizations of initial substrate concentration and reaction duration, the classical initial rate method works when an estimated initial rate locates within the linear range; otherwise, kinetic analysis of reaction curve applies after the conversion of the quantification index with optimized parameters.
This integration strategy has ideal linear ranges and practical efficiency for quantifying an enzyme at moderate substrate levels and for quantifying a substrate at moderate cost on tool enzyme; it has promise to simultaneous assays of multiple enzymes in one reaction vessel each time and ,thus, potential applications to concurrently quantify multiple serum enzymes, screen inhibitors against multiple enzyme targets, and detect multiple serum components by enzymeimmunoassay.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yang, Xiaolan& Long, Gaobo& Jiang, Hairong& Liao, Pu& Liao, Fei. 2012. Integration of Kinetic Analysis of Reaction Curve with a Proper Classical Approach for Enzymatic Analysis. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-512303
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yang, Xiaolan…[et al.]. Integration of Kinetic Analysis of Reaction Curve with a Proper Classical Approach for Enzymatic Analysis. The Scientific World Journal No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-512303
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yang, Xiaolan& Long, Gaobo& Jiang, Hairong& Liao, Pu& Liao, Fei. Integration of Kinetic Analysis of Reaction Curve with a Proper Classical Approach for Enzymatic Analysis. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-512303
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-512303
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر