Molecular Cloning and Characterization of P4 Nuclease from Leishmania infantum
المؤلفون المشاركون
Maleki zanjani, Bahram
Beh-pajooh, Abbas
Montazer Saheb, Soheila
Abdoli Oskoee, Shahin
Alimohammadian, Mohammad Hossein
Saeedi, Nazli
Rahbarnia, Leila
Farajnia, Safar
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-06-28
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Parasite of the genus Leishmania is reliant on the salvage pathway for recycling of ribonucleotides.
A class I nuclease enzyme also known as P4 nuclease is involved in salvage of purines in cutaneous Leishmania species but the relevant enzymes have not been characterized in Leishmania infantum (L.
infantum).
The aim of this study was to clone and characterize the gene encoding class I nuclease in L.
infantum.
DNA extracted from L.
infantum was used for amplification of P4 nuclease gene (Li-P4) by PCR.
The product was cloned, sequenced, and expressed in E.
coli for further characterization.
Analysis of the sequence of Li-P4 revealed that the gene consists of an ORF of 951 bp.
Sequence similarity analysis indicated that Li-P4 has a high homology to relevant enzymes of other kintoplastids with the highest homology (88%) to p1/s1 class I nuclease from L.
donovani.
Western blotting of antirecombinant Li-P4 with promastigote and amastigote stages of L.
infantum showed that this nuclease is present in both stages of parasite with higher expression in amastigote stage.
The highly conserved nature of this essential enzyme in Leishmania parasites suggests it as a promising drug target for leishmaniasis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Farajnia, Safar& Rahbarnia, Leila& Maleki zanjani, Bahram& Alimohammadian, Mohammad Hossein& Abdoli Oskoee, Shahin& Beh-pajooh, Abbas…[et al.]. 2011. Molecular Cloning and Characterization of P4 Nuclease from Leishmania infantum. Enzyme Research،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-512420
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Farajnia, Safar…[et al.]. Molecular Cloning and Characterization of P4 Nuclease from Leishmania infantum. Enzyme Research No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-512420
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Farajnia, Safar& Rahbarnia, Leila& Maleki zanjani, Bahram& Alimohammadian, Mohammad Hossein& Abdoli Oskoee, Shahin& Beh-pajooh, Abbas…[et al.]. Molecular Cloning and Characterization of P4 Nuclease from Leishmania infantum. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-512420
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-512420
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر