P90RSK and Nrf2 Activation via MEK12-ERK12 Pathways Mediated by Notoginsenoside R2 to Prevent 6-Hydroxydopamine-Induced Apoptotic Death in SH-SY5Y Cells
المؤلفون المشاركون
Sun, Xiao-Bo
Sun, Jing
Wang, Min
Sun, Gui-Bo
Meng, Xiang-Bao
Qin, Meng
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-18
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
6-Hydroxydopamine (6-OHDA) is known to contribute to neuronal death in Parkinson's disease.
In this study, we found that the preincubation of SH-SY5Y cells for 24 h with 20 μM notoginsenoside R2 (NGR2), which is a newly isolated notoginsenoside from Panax notoginseng, showed neuroprotective effects against 6-OHDA-induced oxidative stress and apoptosis.
NGR2 incubation successively resulted in the activation of P90RSK, inactivation of BAD, and inhibition of 6-OHDA-induced mitochondrial membrane depolarization, thus preventing the mitochondrial apoptosis pathway.
NGR2 incubation also led to the activation of Nrf2 and subsequent activity enhancement of phase II detoxifying enzymes, thus suppressing 6-OHDA-induced oxidative stress, and these effects could be removed by Nrf2 siRNA.
We also found that the upstream activators of P90RSK and Nrf2 were the MEK1/2–ERK1/2 pathways but not the JNK, P38, or PI3K/Akt pathways.
Interestingly, NGR2 incubation could also activate MEK1/2 and ERK1/2.
Most importantly, NGR2-mediated P90RSK and Nrf2 activation, respective downstream target activation, and neuroprotection were reversed by the genetic silencing of MEK1/2 and ERK1/2 by using siRNA and PD98059 application.
These results suggested that the neuroprotection elicited by NGR2 against 6-OHDA-induced neurotoxicity was associated with NGR2-mediated P90RSK and Nrf2 activation through MEK1/2-ERK1/2 pathways.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Meng, Xiang-Bao& Sun, Gui-Bo& Wang, Min& Sun, Jing& Qin, Meng& Sun, Xiao-Bo. 2013. P90RSK and Nrf2 Activation via MEK12-ERK12 Pathways Mediated by Notoginsenoside R2 to Prevent 6-Hydroxydopamine-Induced Apoptotic Death in SH-SY5Y Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-512478
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Meng, Xiang-Bao…[et al.]. P90RSK and Nrf2 Activation via MEK12-ERK12 Pathways Mediated by Notoginsenoside R2 to Prevent 6-Hydroxydopamine-Induced Apoptotic Death in SH-SY5Y Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-512478
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Meng, Xiang-Bao& Sun, Gui-Bo& Wang, Min& Sun, Jing& Qin, Meng& Sun, Xiao-Bo. P90RSK and Nrf2 Activation via MEK12-ERK12 Pathways Mediated by Notoginsenoside R2 to Prevent 6-Hydroxydopamine-Induced Apoptotic Death in SH-SY5Y Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-512478
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-512478
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر