Rapid, Simultaneous Detection of Clostridium sordellii and Clostridium perfringens in Archived Tissues by a Novel PCR-Based Microsphere Assay : Diagnostic Implications for Pregnancy-Associated Toxic Shock Syndrome Cases
المؤلفون المشاركون
Bhatnagar, Julu
Kellar, Kathryn L.
Bandyopadhyay, Kakali
Zaki, Sherif R.
Antoniadou, Zoi-Anna
Paddock, Christopher D.
DeLeon-Carnes, Marlene
Shieh, Wun-Ju
المصدر
Infectious Diseases in Obstetrics and Gynecology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-03-22
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Clostridium sordellii and Clostridium perfringens are infrequent human pathogens; however, the case-fatality rates for the infections are very high, particularly in obstetric C.
sordellii infections (>90%).
Deaths from Clostridium sordellii and Clostridium perfringens toxic shock (CTS) are sudden, and diagnosis is often challenging.
Formalin-fixed, paraffin-embedded (FFPE) tissues usually are the only specimens available for sudden fatal cases, and immunohistochemistry (IHC) for Clostridia is generally performed but it cannot identify species.
A clear need exists for a rapid, species-specific diagnostic assay for FFPE tissues.
We developed a duplex PCR-based microsphere assay for simultaneous detection of C.
sordellii and C.
perfringens and evaluated DNA extracted from 42 Clostridium isolates and FFPE tissues of 28 patients with toxic shock/endometritis (20 CTS, 8 non-CTS, as confirmed by PCR and sequencing).
The microsphere assay correctly identified C.
sordellii and C.
perfringens in all known isolates and in all CTS patients (10 C.
sordellii, 8 C.
perfringens, 2 both) and showed 100% concordance with PCR and sequencing results.
The microsphere assay is a rapid, specific, and cost-effective method for the diagnosis of CTS and offers the advantage of simultaneous testing for C.
sordellii and C.
perfringens in FFPE tissues using a limited amount of DNA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Bhatnagar, Julu& DeLeon-Carnes, Marlene& Kellar, Kathryn L.& Bandyopadhyay, Kakali& Antoniadou, Zoi-Anna& Shieh, Wun-Ju…[et al.]. 2012. Rapid, Simultaneous Detection of Clostridium sordellii and Clostridium perfringens in Archived Tissues by a Novel PCR-Based Microsphere Assay : Diagnostic Implications for Pregnancy-Associated Toxic Shock Syndrome Cases. Infectious Diseases in Obstetrics and Gynecology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-512602
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Bhatnagar, Julu…[et al.]. Rapid, Simultaneous Detection of Clostridium sordellii and Clostridium perfringens in Archived Tissues by a Novel PCR-Based Microsphere Assay : Diagnostic Implications for Pregnancy-Associated Toxic Shock Syndrome Cases. Infectious Diseases in Obstetrics and Gynecology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-512602
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Bhatnagar, Julu& DeLeon-Carnes, Marlene& Kellar, Kathryn L.& Bandyopadhyay, Kakali& Antoniadou, Zoi-Anna& Shieh, Wun-Ju…[et al.]. Rapid, Simultaneous Detection of Clostridium sordellii and Clostridium perfringens in Archived Tissues by a Novel PCR-Based Microsphere Assay : Diagnostic Implications for Pregnancy-Associated Toxic Shock Syndrome Cases. Infectious Diseases in Obstetrics and Gynecology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-512602
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-512602
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر