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Improvement of the Digestibility of Sulfated Hyaluronans by Bovine Testicular Hyaluronidase : A UV Spectroscopic and Mass Spectrometric Study
المؤلفون المشاركون
Lemmnitzer, Katharina
Schiller, Jürgen
Becher, Jana
Möller, Stephanie
Schnabelrauch, Matthias
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-27
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Glycosaminoglycans (GAGs) such as hyaluronan (HA) and chondroitin sulfate (CS) are important, natural polysaccharides which occur in biological (connective) tissues and have various biotechnological and medical applications.
Additionally, there is increasing evidence that chemically (over)sulfated GAGs possess promising properties and are useful as implant coatings.
Unfortunately, a detailed characterization of these GAGs is challenging: although mass spectrometry (MS) is one of the most powerful tools to elucidate the structures of (poly)saccharides, MS is not applicable to high mass polysaccharides, but characteristic oligosaccharides are needed.
These oligosaccharides are normally generated by enzymatic digestion.
However, chemically modified (particularly sulfated) GAGs are extremely refractive to enzymatic digestion.
This study focuses on the investigation of the digestibility of GAGs with different degrees of sulfation by bovine testicular hyaluronidase (BTH).
It will be shown by using an adapted spectrophotometric assay that all investigated GAGs can be basically digested if the reaction conditions are carefully adjusted.
However, the oligosaccharide yield correlates reciprocally with the number of sulfate residues per polymer repeating unit.
Finally, matrix-laser desorption and ionization (MALDI) MS will be used to study the released oligosaccharides and their sulfation patterns.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lemmnitzer, Katharina& Schiller, Jürgen& Becher, Jana& Möller, Stephanie& Schnabelrauch, Matthias. 2014. Improvement of the Digestibility of Sulfated Hyaluronans by Bovine Testicular Hyaluronidase : A UV Spectroscopic and Mass Spectrometric Study. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-513825
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lemmnitzer, Katharina…[et al.]. Improvement of the Digestibility of Sulfated Hyaluronans by Bovine Testicular Hyaluronidase : A UV Spectroscopic and Mass Spectrometric Study. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-513825
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lemmnitzer, Katharina& Schiller, Jürgen& Becher, Jana& Möller, Stephanie& Schnabelrauch, Matthias. Improvement of the Digestibility of Sulfated Hyaluronans by Bovine Testicular Hyaluronidase : A UV Spectroscopic and Mass Spectrometric Study. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-513825
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-513825
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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